Box-Behnken设计优化石斛清咽含片提取工艺  

Optimization of Extraction Technology of Shihu Qingyan Lozenges by Box-Behnken Design-response Surface Methodology

作　　者：刘怀伟[1] 邹俊波[1] 张廷模[1] 邹文俊[1] 

机构地区：[1]成都中医药大学药学院,成都611137

出　　处：《中国实验方剂学杂志》2014年第21期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011BAI13B02-8)

摘　　要：目的：优选石斛清咽含片的提取工艺.方法：采用HPLC测定石斛酚含量,检测波长278 nm,流动相乙腈（A）-0.5％甲酸（B）梯度洗脱（0 - 20 min,27％A;20 - 30 min,27％ - 40％A;30- 45 min,40％ - 65％A;45 - 50 min,65％ - 27％A）.以石斛酚提取量和干膏率的综合评分为指标,在单因素试验基础上,通过Box-Behnken试验考察提取时间、料液比、浸泡时间对提取工艺的影响.结果：最佳提取工艺为加11倍量水浸泡35 min,提取3次,每次1h;石斛酚提取量0.189 6 mg·g-1,干膏得率41.27％.结论：通过Box-Behnken效应面法及总评“归一值”为评价系统优选的提取工艺合理可行,为石斛清咽含片的应用提供参考.Objective：To optimize extraction process of Shihu Qingyan lozenges by Box-Behnken design-response surface methodology.Method：HPLC was employed to determine the content of dendrophnol with detection wavelength at 278 nm and mobile phase of acetonitrile （A）-0.5％ formic acid （B） for gradient elution （0-20 min,27％A; 20-30 min,27％-40％A; 30-45 min,40％-65％A; 45-50 min,65％-27％A）.On the basis of single factor tests,a three-factor and three-level Box-Behnken experimental design was employed to investigate effects of extraction time,solid-liquid ratio and soaking time on composite score of extracting amount of dendrophnol and dry extract rate.Result：Optimum process conditions were as follows：soaked 35 min with 11 times the amount of water,extracted thrice for 1 h each time; extracting amount of dendrophnol was 0.189 6 mg ·g-1 and dry extract rate was 41.27％.Conclusion：This optimized extraction technology is practical and rational by BoxBehnken design-response surface methodology and overall desirability,it provides a reference for application of Shihu Qingyan lozenges.

关 键 词：石斛清咽含片 BOX-BEHNKEN设计 总评“归一值” 桔梗 叠鞘石斛 石斛酚 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]