维吾尔族药毛菊苣总倍半萜滴丸溶出度测定  

Determination of Dissolution of Total Sesquiterpenes from Cichorium glandulosum Dropping Pills

作　　者：罗玉琴[1] 杨伟俊 信学雷[1] 阿吉艾克拜尔·艾萨[1] 

机构地区：[1]中国科学院干旱区植物资源化学重点实验室,新疆特有药用资源利用国家重点实验室培育基地,中国科学院新疆理化技术研究所,乌鲁木齐830011 [2]新疆维吾尔自治区药物研究所新疆维吾尔药重点实验室,乌鲁木齐830004

出　　处：《中国实验方剂学杂志》2014年第21期21-24,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81303307)

摘　　要：目的：建立毛菊苣总倍半萜滴丸中山莴苣素的溶出度测定方法.方法：采用桨法测定体外溶出度,通过单因素试验筛选溶出介质和转速,利用HPLC测定毛菊苣总倍半萜滴丸中山莴苣素的溶出度,流动相甲醇（A）-0.2％甲酸（B）梯度洗脱（0 - 30 min,28％- 72％ A）,检测波长256 nm.结果：选择含0.2％十二烷基硫酸钠的人工胃液为溶出介质,转速100r·min-1,山莴苣素线性范围0.015 -0.075 g·L-1（r=0.999）,平均回收率101.04％ （RSD 1.38％）.样品30 min时体外溶出度均＞80％.结论：本方法简便、准确、可靠,适用于毛菊苣总倍半萜滴丸的溶出度测定.Objective：To establish a method for determining dissolution of lactucin in total sesquiterpenes from Cichorium glandulosum dropping pills.Method：Paddle method was adopted in which artificial gastric juice was taken as solvent with a rotating speed of 100 r · min-1 Dissolution of lactucin was determined by HPLC and dissolution curves were drawn,mobile phase is made up of methanol （A）-0.2％ formic acid （B） for gradient elution （0-30 min,28％-72％ A） and detection wavelength was set at 256 nm.Result：Lactucin was linear over the range of 0.015-0.075 g ·L-1 with r of 0.999 and average recovery was 101.04％ with RSD of 1.38％.Taking artificial gastric juice containing 0.2％ sodium dodecyl sulfate as dissolution medium,dissolution of samples were all more than 80％ in 30 min.Conclusion：This method is simple,accurate and reliable for dissolution determination of total sesquiterpenes from Cichorium glandulosum dropping pills.

关 键 词：毛菊苣 总倍半萜 溶出度 山莴苣素 

分 类 号：R283.6[医药卫生—中药学] R287[医药卫生—中医学]