地龙渗漉工艺参数优选  

Optimization of Percolation Process Parameters of Pheretima

作　　者：徐玉玲[1] 谭清红 伍利华[3] 王晓霞[4] 吴南轩 梁悦[4] 刘涛[4] 

机构地区：[1]成都大学实验技术中心,成都610106 [2]四川科伦药物研究院有限公司,成都610000 [3]广西中医药大学,南宁530001 [4]成都大学生物产业学院,成都610106

出　　处：《中国实验方剂学杂志》2014年第21期41-43,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省卫生厅科研课题(130406)

摘　　要：目的：优选地龙的渗漉工艺参数.方法：采用体外溶血法,以含固量和蛋白酶活性为综合评价指标,通过单因素试验优选地龙的渗漉工艺.结果：尿激酶在25 -125 U·mL-1与溶圈面积呈良好线性关系,最佳渗漉工艺参数为地龙粉碎为粗粉,浸泡时间0.5h,渗漉速度2 mL·min-1,加8倍量60％乙醇渗漉;渗漉液体积220 mL,含固量3.252 g,蛋白酶活性5 624.7 U·g-1.结论：纤维蛋白原平板法可快速、简便、准确地测定地龙中酶的活性,优选的工艺参数稳定可行,为地龙有效成分的充分利用提供新选择.Objective：To optimize percolation parameters of Pheretima.Method：By using external hemolysis method,with solid content and protease activity as indicators,single factor tests were adopted to optimize percolation process.Result：The concentration of urokinase in 25-125 U ·mL-1 showed a good linear relationship with soluble circle area,the best percolation processing parameters were：crushed Pheretima to a coarse powder,soaked 0.5 h with percolation rate of 2 mL ·min-1,added 8 times the amount of 60％ ethanol for percolation; percolate volume,solid content and protease activity were 220 mL,3.252 g,5 624.7 U ·g-1 Conclusion：Fibrinogen plate method can be fast,easy and accurate to determine enzyme activity in Pheretima,these optimized process parameters was stable and feasible,it provides new choice for full utilization of active ingredients from Pheretima.

关 键 词：地龙 酶活性 渗漉工艺 纤维蛋白原平板法 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]