动态优化方法对维C银翘片中牛蒡子提取时间的优化  

Application of Dynamic Optimization Method for Extraction Time of Arctii Fructus in Vitamin C Yinqiao Pian

作　　者：查钦 杨世林[2] 章军[1] 陈莎[1] 王跃生[1] 刘安[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]江西中医药大学,南昌330004 [3]毕节市中药研究所,贵州毕节551700

出　　处：《中国实验方剂学杂志》2014年第21期44-46,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI13B04)

摘　　要：目的:采用动态优化方法优选维C银翘片中牛蒡子的提取工艺。方法:采用HPLC测定牛蒡子苷含量,检测波长280 nm,流动相乙腈-水(20∶80)。通过比较动态优化工艺与原提取工艺对维C银翘片提取率、指标成分含量和指纹图谱的相似度,评价动态优化方法应用于维C银翘片中牛蒡子提取工艺优化的可行性。结果:动态优化工艺与原提取工艺所得提取物基本一致。动态优化工艺为加8倍量60%乙醇回流提取2次,提取时间分别为140,60 min;提取时间较原工艺节约58%,耗电量节约53%。结论:动态优化方法可运用于维C银翘片中牛蒡子提取工艺的优化,该工艺能减少提取时间、降低能耗,具有明显的经济效益。Objective：To optimize extraction process of Arctii Fructus in Vitamin C Yinqiao Pian by dynamic optimization extraction method （DOEM）.Method：HPLC was employed to determine the content of arctiin with detection wavelength at 280 nm and mobile phase of acetonitrile-water （20∶ 80）.Taking yield and content of arctiin,fingerprints similarity as indexes,feasibility of DOEM using in Arctii Fructus from Wei C Yinqiao tablets was evaluated,and compared with extraction process in 2010 edition of .Result：There was no difference in extract between dynamic optimization process and ordinary extraction process.Optimized DOEM was as following：reflux extracted twice with 8 times the amount of 60％ ethanol for extracting time of 140 min and 60 min; it could save 58％ of extraction time and 53％ of power consumption.Conclusion：DOEM can be applied to optimize extraction process of Arctii Fructus in Vitamin C Yinqiao Pian,it can significantly reduce extraction time and production costs.

关 键 词：动态优化法 牛蒡子 牛蒡子苷 提取时间 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]