左、右归丸及其拆方中马钱苷的变化规律  

Variation of Loganin in Zuogui Wan,Yougui Wan and Their Disassembled Prescriptions

作　　者：杜丽霞[1,2] 何文智[1] 刘晓阳[1] 王智民[3] 任艳玲[1] 

机构地区：[1]辽宁中医药大学,沈阳110847 [2]哈尔滨市中医医院,哈尔滨150076 [3]辽宁中医药大学第一临床学院,沈阳110847

出　　处：《中国实验方剂学杂志》2014年第21期56-59,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81373527)

摘　　要：目的：建立左、右归丸及其拆方的马钱苷含量测定方法,为左、右归丸及其拆方质量控制提供标准.方法：采用HPLC法,ZOREAX SB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-水梯度洗脱,流速1.0 mL· min-1,检测波长240 nm,进样量20μL,柱温30 ℃.结果：所建方法能较好测定马钱苷的含量变化,出峰位置前后均无杂峰干扰,峰形完整,马钱苷在0.2890 -7.225 0 μg（r =0.999 6）呈良好的线性关系,平均回收率为99.70％,RSD 1.5％.稳定性、精密度和加样回收率均符合定量要求,含量测定结果显示马钱苷在共同药方中含量最高,右归丸和滋肾阴药中含量相似,左归丸中含量偏低.结论：该方法简便、重复性好,结果准确可靠,可为左、右归丸及其拆方的质量控制提供参考价值和指导意义.Objective：To set up a HPLC method for the determination of loganin in Zuogui Wan,Yougui Wan and their disassembled prescriptions.It can provide a quality control standard for Zuogui Wan,Yougui Wan and their disassembled prescriptions.Method：HPLC,a ZOREAX SB-C18 column served as the solid phase,the mobile phase was acetonitrile-water in gradient elution mode.The flow rate was 1.0 mL ·min-1 and the sample volume was 20 μL each time,the detection wave length was 240 nm and the column temperature was 30 ℃.Result：The topic establishing method can better determine the content variation of loganin.Both before and after the peak position without impurity peak interference,and the peak was intact.The linear range of loganin was 0.289 0-7.225 0 μg （r =0.999 6）.The average recoveries were 99.70％,with RSD of 1.5％.Stability,precision and the rate of sample recovery were all well.According to the results,the content of loganin in common prescription was the highest,while it was low in Zuogui Wan.Yougui Wan and kidney-Yin-nourishing drug has a similar level.Conclusion：The method is simple,accurate and reliable with good reproducibility.It can be used to provide the reference value and guiding significance for Zuogui Wan,Yougui Wan and their disassembled prescriptions＇ quality control.

关 键 词：左归丸 右归丸 马钱苷 

分 类 号：R284.1[医药卫生—中药学]