紫金龙乙醇组分对脂多糖诱导的RAW264.7细胞分泌炎症因子的影响  

Effects of Ethanol Componentons from Aconitum vilmorinianum on Inflammatory Cytokines in LPS-stimulated RAW 264.7 Cell

作　　者：张晓红[1] 董莉[1] 杨雅欣[2] 刘星星[1] 廖尚高[2] 董永喜[2] 李勇军[2] 王爱民[2] 

机构地区：[1]贵阳医学院药学院贵州省药物制剂重点实验室,贵阳550004 [2]贵阳医学院民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《中国实验方剂学杂志》2014年第21期149-152,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划课题(2013BAI11B01);贵州省中药现代化项目(黔科合中药字[2011]5028号;黔科合中药字[2013]5062号);贵州省科技计划课题(黔科合LG字[2012]017号)

摘　　要：目的：研究紫金龙乙醇组分（AVEC）对脂多糖（LPS）诱导小鼠单核巨噬细胞RAW 264.7分泌炎症因子的影响.方法：用脂多糖（10 μg· L-1）刺激生长良好的RAW 264.7细胞24 h建立体外细胞炎症模型,以MTT法测定不同浓度AVEC对RAW 264.7细胞的毒性作用,Griess试剂法检测一氧化氮（NO）含量,ELISA法检测细胞上清液中肿瘤坏死因子α（TN F-α）和白介素6（IL-6）含量.结果：AVEC在低于400 mg·L-1时对RAW264.7细胞无毒性作用.与空白对照组相比,LPS可以明显诱导RAW264.7细胞分泌炎症因子TNF-α,IL-6和NO（P＜0.01）;与模型组相比,100 -400 mg·L-1的AVEC可明显下调LPS诱导的RAW264.7细胞释放炎症因子TNF-α,IL-6和NO（P ＜0.05,P＜0.01）,并呈现良好的剂量依赖关系.结论：AVEC可以抑制脂多糖诱导的RAW 264.7细胞炎症反应,其抗炎作用可能与减少炎症因子TNF-α,IL-6和NO有关.Objective：To study the effects of ethanol componentons from Aconitum vilmorinianum （AVEC） on inflammatory cytokines in lipopolysaccharide （LPS）-stimulated macrophage model.Method：The in vitro model of inflammation model based on the growth of well RAW 264.7 cells was established by treating with LPS （10 μg ·L-1） for 24 h.The activities of macrophages were detected by MTT assay.The production of nitric oxide （NO） was assayed by Griess reagent.ELISA were used to assay the production of inflammatory mediators,such as tumor necrosis factor α （TNF-α） and interleukin 6 （IL-6） in cell supernatant.Result：The cell viability was not significantly affected by up to 400 mg ·L-1 of AVEC.Compared with the control group,LPS could induce RAW 264.7 cells to secrete inflammatory mediators like TNF-α,IL-6 and NO （P ＜ 0.01）.Compared with the model group,100-400 mg ·L-1 of AVEC in LPS-stimulated RAW 264.7 cells greatly inhibited the production of inflammatory mediators,such as TNF-α and IL-6 in a good dose dependent manner （P ＜ 0.05,P ＜ 0.01）.Conclusion：AVCE can inhibit LPS-induced inflammatory response in RAW 264.7 cells,and its anti-inflammatory effect may be related to reducing the inflammatory cytokines like TNF-α,IL-6 and NO.

关 键 词：紫金龙 脂多糖 肿瘤坏死因子Α 白介素6 

分 类 号：R285.5[医药卫生—中药学]