白屈菜红碱对小鼠溃疡性结肠炎的治疗作用  

Effect of Chelerythrine on Ulcerative Colitis in Mice

作　　者：徐香琴[1] 黄松[1] 杜先华[1] 周家乐[1] 黄火娣 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2014年第21期171-174,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2012BAI29B00)

摘　　要：目的:研究白屈菜红碱对小鼠溃疡性结肠炎的治疗作用及可能的作用机制。方法:选取60只KM小鼠采用乙酸灌肠建立小鼠溃疡性结肠炎模型,随机分为模型组,阳性药组,白屈菜红碱低、中、高剂量组(25,50,100 mg·kg-1),并设立正常组,每组10只。灌胃给药,正常组、模型组给予生理盐水;阳性药组给予20 mg·kg-1美沙拉嗪。连续给药7 d后,观察白屈菜红碱对小鼠一般情况、结肠黏膜组织损伤的影响;试剂盒测结肠和血清中髓过氧化物酶(MPO)的活性和一氧化氮(NO)的含量;酶联免疫吸附法(ELISA)检测结肠和血清中肿瘤坏死因子-α(TNF-α)水平变化。结果:与正常组相比,模型组结肠组织损伤严重,MPO,NO,TNF-α的水平明显降低(P<0.01);与模型组相比,阳性药组和白屈菜红碱组结肠组织损伤情况明显减轻,美沙拉嗪与白屈菜红碱可改善升高MPO,NO,TNF-α水平(P<0.05,P<0.01)。结论:白屈菜红碱对乙酸诱导的小鼠溃疡性结肠炎有治疗作用,作用机制可能与其炎症因子NO,TNF-α的产生有关。Objective：To investigate the therapeutic effect of chelerythrine on experimental ulcerative colitis and possible mechanism.Method：Acetic acid-induced ulcerative colitis （UC） mice model was prepared.60 mice were randomized into model group,positive group,chelerythrine low,medium and high dose groups （25,50,100 mg ·kg-1）,and a normal group was set up,10 mice was included in each group.The general condition and pathological changes were observed.Myeloperoxidase （MPO） activity and nitric oxide （NO） levels in colon tissue and serum were determined by biochemical assay.Tumor necrosis factor （TNF-α） levels in colon tissue and serum were detected by enzyme-linked immunosorbent assay （ELISA）.Result：Compared with the normal group,the injury of colonic tissue in model group was serious,the level of MPO,NO,TNF-α was significantly lower （P ＜0.01）.Compared with the model group,chelerythrine and etiasa significantly alleviated the damage of colonic tissue.The activities of MPO and NO,TNF-α levels were also obviously decreased （P ＜0.05,P ＜ 0.01）.Conclusion：Chelerythrine may play its therapeutic role through inhibiting the inflammation due to the generation of NO,TNF-α.

关 键 词：白屈菜红碱 溃疡性结肠炎 髓过氧化物酶 一氧化氮 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]