高脂诱导糖耐量低减大鼠模型骨骼肌中相关蛋白的表达  

Experiment on Protein Expression in Skeletal Muscle of Rats with Impaired Glucose Tolerance

作　　者：姜广坤[1] 肖洪彬[1] 李凤金[1] 牛雯颖[1] 李雨庭[1] 宋颖星 张广庆[1] 郑慧娟[1] 马伯艳[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国实验方剂学杂志》2014年第21期183-186,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(81102545);黑龙江省自然科学基金(D201263);黑龙江中医药大学优秀创新人才计划项目

摘　　要：目的:研究45%高脂饲料长程喂养复制糖耐量低减(IGT)大鼠骨骼肌中磷脂酰肌醇3激酶(PI3K),蛋白激酶B(PKB),糖原合成酶激酶3(GSK-3β)、葡萄糖转运蛋白4(GLUT-4)的蛋白表达,探讨该模型产生糖耐量低减的分子机制。方法:20只SD大鼠随机分为2组,分别为正常组和模型组,正常组给予10%热量比对照饲料,模型组给予45%热量比高脂饲料喂养20周建立IGT大鼠模型,分别取骨骼肌采用免疫组化法测定PI3K蛋白含量,Western blot法检测PKB,GSK-3β,GLUT-4蛋白表达,Real Time-PCR法检测IGT大鼠骨骼肌中GLUT-4蛋白相对含量。结果:与正常组比较,IGT组骨骼肌PI3K,PKB,蛋白表达均显著降低(P<0.01),GSK-3β蛋白表达明显升高(P<0.01),IGT模型大鼠骨骼肌中GLUT-4 mRNA的表达降低,但与正常组比较无统计学差异。结论:45%高脂饲料长程喂养建立的IGT模型骨骼肌PI3K,PKB,GSK-3β,GLUT-4蛋白表达发生明显的变化,这可能是该种造模方式导致IGT发生的分子机制之一。Objective：To discusse the molecular mechanisms of impaired glucose tolerance （IGT） development induced by long term high-fat diet by investigating the protein expressions of phosphatidyl inositol 3-kinase （PI3K） /protein kinase B （PKB） /glycogen synthase kinase-3β （GSK-3β） /glucose transporter-4 （GLUT-4） in the rat skeletal muscle with IGT.Method：The IGT rat model was replicated by being fed with high fat diet with 45％ lipid energy for twenty weeks.The protein concentration of PI3K in the skeletal muscle was examined by immunohistochemical staining,the protein expressions of PKB/GSK-3β/GLUT-4 were detected by Western Blot,and the relative amount of GLUT-4 protein in IGT rats skeletal muscle tissue was tested by Real Time-PCR.Result：Compared to the control group,the protein expressions of PI3K/PKB in skeletal muscle of IGT group were all decreased,the protein expressions of GSK-3β was obvously increased （P ＜0.01）.The mRNA expression of GLUT-4 in IGT group was decreased as compared with the control group,but there was no significant difference.Conclusion：The protein expressions of PKB/GSK-3β/GLUT-4 in skeletal muscle of IGT rats established by long-term high fat diet with 45％ lipid energy are obviously changed,which seems to be one of the molecular mechanisms for IGT in this model formation.

关 键 词：糖耐量低减 磷脂酰肌醇3激酶 蛋白激酶B 糖原合成酶激酶3 葡萄糖转运蛋白4 

分 类 号：R285.5[医药卫生—中药学]