电针丰隆穴对高脂血症大鼠腹腔巨噬细胞CD11b、ICAM-1表达的影响  

Effect of Electroacupuncture at Point Fenglong on the Expressions of Peritoneal Macrophage CD11b and ICAM-1 in Hyperlipidemia Rats

作　　者：王琼[1] 田佳玉[1] 肖颖[1] 张红星[1] 

机构地区：[1]武汉市中西医结合医院,武汉430022

出　　处：《上海针灸杂志》2014年第10期951-954,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(30873309)

摘　　要：目的观察电针丰隆穴对高脂血症大鼠巨噬细胞表面抗原CD11b、细胞间黏附分子-1(ICAM-1)表达的影响。方法将40只健康SD大鼠随机分为正常对照组、高脂饲料组、高脂+普通饲料组、高脂饲料治疗组及高脂+普通饲料治疗组。电针丰隆穴治疗28 d后,检测各组大鼠血脂水平,即总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量,流式细胞术(flow cytometry,FCM)检测各组大鼠腹腔巨噬细胞表面抗原CD11b、ICAM-1表达。结果高脂饲料组大鼠血浆TC、LDL-C较正常对照组明显上升(P<0.01);高脂+普通饲料组大鼠血清TC、LDL-C与高脂饲料组比较明显下降(P<0.01),较正常对照组仍升高明显(P<0.01);电针丰隆穴治疗后,大鼠血浆TC、LDL-C明显下降(P<0.01);TG、HDL-C变化不明显(P>0.05)。高脂饲料组大鼠巨噬细胞CD11b、ICAM-1表达率较正常对照组明显上升(P<0.01),高脂+普通饲料组大鼠巨噬细胞CD11b、ICAM-1表达率较高脂饲料组明显下降(P<0.05),较正常对照组仍明显上升(P<0.01),高脂饲料治疗组与高脂饲料组比较,CD11b、ICAM-1表达率明显下降(P<0.01),高脂+普通饲料治疗组与高脂+普通饲料组比较,CD11b、ICAM-1表达率明显降低(P<0.01),高脂+普通饲料治疗组与高脂饲料治疗组比较,CD11b、ICAM-1表达率明显降低(P<0.01)。相关分析显示,CD11b与ICAM-1水平呈显著正相关(r=0.947,P<0.01)。结论电针丰隆穴能够明显下调高脂血症大鼠血脂中TC、LDL-C水平,下调高脂血症大鼠巨噬细胞CD11b、ICAM-1的表达,对高脂血症具有一定的治疗作用。Objective To investigate the effect of electroacupuncture at point Fenglong（ST40） on the expressions of macrophage surface antigen CD11b and intercellular adhesion molecule-1 （ICAM-1） in hyperlipidemia rats. Method Forty healthy SD rats were randomly allocated to normal control, high fat diet, high fat＋common diet, high fat diet and treatment, and high fat＋common diet and treatment groups. After 28 days of treatment with electroacupuncture at point Fenglong, blood lipid levels, namely, total cholesterol （TC）, triglyceride （TG）, low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C） contents were measured in every group of rats. The expressions of peritoneal macrophage surface antigen CD11b and ICAM-1 were examined using flow cytometry （FCM） in every group of rats. Result Plasma TC and LDL-C increased significantly in the high fat diet group of rats compared with the normal control group （P〈0.01）. In the high fat＋common diet group of rats, serum TC and LDL-C decreased significantly in comparison with the high fat diet group （P〈0.01） but increased significantly in comparison with the normal control group （P〈0.01）. After treatment with electroacupuncture at point Fenglong, rat plasma TC and LDL-C decreased significantly （P〈0.01） and TG and HDL-C did not change significantly （P〉0.05）. The expression rates of macrophage CD11b and ICAM-1 increased significantly in the high fat diet group of rats compared with the normal group （P〈0.01）. In the high fat＋common diet group of rats, the expression rates of macrophage CD11b and ICAM-1 decreased significantly in comparison with the high fat diet group （P〈0.05） but increased significantly in comparison with the normal group （P〈0.01）. The expression rates of CD11b and ICAM-1 decreased significantly in the high fat diet and treatment group compared with the high fat diet group （P〈0.01） and decreased significantly in the high fat＋common diet and treatm

关 键 词：高脂血症 电针 穴 丰隆 巨噬细胞 CD11B ICAM-1 大鼠 

分 类 号：R2-03[医药卫生—中医学]