穿心莲生理特性及盐胁迫对其影响研究  

Study on Physiological Characteristics and Effects of Salt Stress in Andrographis paniculata

作　　者：陈娟[1] 谷巍[1] 段金廒[1] 宿树兰[1] 邵婧[1] 耿超[1] 

机构地区：[1]南京中医药大学药学院/江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地主联合工程研究中心,江苏南京210023

出　　处：《中药材》2014年第8期1322-1327,共6页Journal of Chinese Medicinal Materials

基　　金：十二五国家科技支撑项目(2012BAI29B02);江苏省"青蓝工程"(2012);江苏省中药优势学科Ⅱ期建设(ysxk-2014);中医药行业科研专项(201407002)

摘　　要：目的:研究穿心莲的生理特性及盐胁迫的影响。方法:用LI-6400光合仪等便携式仪器及试剂盒等研究穿心莲的光合、蒸腾、酶活等生理特性,用不同浓度NaCl对穿心莲进行盐胁迫处理,并测定各生理指标的变化。结果:穿心莲净光合速率(Pn)与气孔导度(Gs)日变化呈双峰曲线,蒸腾速率(Tr)及气孔限制值(Ls)日变化呈单峰曲线,胞间CO2浓度(Ci)及水分利用率(WUE)日变化呈单谷曲线。盐胁处理下,随着浓度的增加,Pn、Tr、Ci、Gs、WUE均下降,Ls呈上升趋势,超氧化物歧化酶、过氧化氢酶和过氧化物酶酶活性均先升后降,丙二醛含量、脯氨酸含量呈上升趋势。结论:穿心莲为阳生植物,存在明显的"光合午休"现象,具一定耐盐性,应向阳种植,并可对其进行适当的遮荫处理,以提高其质量。Objective：To study the physiological characteristics and effects of salt stress in Andrographis paniculata. Methods：Andrographis paniculata was treated with NaCl of different concentration. The photosynthetic characteristics and transpiration rate were analyzed by LI-6400 Portable Photosynthesis System. The activities of enzymes were studied with kits. Results：The net photosynthetic rate（Pn）and stomatal conductance（Gs）showed a diurnal variation of bimodal curve,the transpiration rate（Tr）and stomatal limitation（Ls）both had a single peak diurnal variation,while the intercellular CO2concentration（Ci）and the water use efficiency（WUE） presented a single valley type of diurnal variation. With salt concentration rising,Pn,Tr,Ci,Caand WUE decreased but Lsincreased,the activities of SOD,CAT and POD increased firstly and then decreased,while the MDA and proline content showed a rising trend. Conclusion：Andrographis paniculata is a type of sun plant. The net photosynthetic rate of Andrographis paniculata leaves has an obvious＂midday depression＂phenomenon. The results also indicate that Andrographis paniculata has a resistance to salt stress and appropriate shade is good for the quality improvement.

关 键 词：穿心莲 生理特性 保护酶系统 渗透调节物质 盐胁迫 

分 类 号：R282.2[医药卫生—中药学]