毛茛总苷对大鼠离体心脏缺血-再灌注损伤的保护作用  

Protective Effect of Total Glycosides of Ranunculus japonicus on Myocardial Ischemic-Reperfusion Injury in Isolated Rat Hearts

作　　者：高啸巍 刘苑[1] 杨智承[1] 谭毓治[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《中药材》2014年第8期1429-1433,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:观察毛茛总苷对大鼠离体心肌缺血-再灌注损伤的保护作用。方法:将SD大鼠随机分为正常对照组、缺血-再灌注损伤模型组及毛茛总苷0.05、0.10、0.20 mg/mL组。采用Langendroff大鼠离体心脏灌流系统,建立离体心脏缺血-再灌注损伤模型。记录停灌前、再灌注后1、5、10、20、30、40 min时的心率(HR)、左室收缩压峰值(LVSP)、左室收缩压最大上升速率(+dp/dtmax)、左室舒张压最大下降速率(-dp/dtmax)、冠脉流量(CF)等心功能指标;测定再灌注后20、40 min灌流液中乳酸脱氢酶(LDH)、肌酸激酶(CK)的活性;采用氯化三苯基四氮唑(TTC)进行心肌染色并计算心肌梗死面积百分比。结果:模型组再灌注后HR、LVDP、±dp/dtmax、CF显著降低,LDH、CK水平升高,心肌梗死面积达58.78%,毛茛总苷对以上指标均有良好的改善作用。结论:毛茛总苷对缺血-再灌注导致的心肌损伤具有良好的保护作用。Objectives：To study the protective effect of total glycosides of Ranunculus japonicus（TGRJ） on myocardial ischemicreperfusion injury in isolated rat hearts. Methods：The SD rats were randomly divided into normal control group,ischemia-reperfusion group,and TGRJ in 0. 05,0. 10 and 0. 20 mg /mL groups. The ischemia-reperfusion injury model was built using Langendroff isolated rats hearts perfusion system. The indexes of heart function such as heart rate（HR）,left ventricular systolic pressure（LVSP）,left ventricular systolic pressure maximum rate of rise（ ＋ dp /dtmax）,left ventricular diastolic pressure maximum rate of descent（-dp/dtmax）and coronary flow（CF）before ischemia and later at 1,5,10,20,30 and 40 min after reperfusion were recorded. The activity of Lactate dehydrogenase（LDH） and creatine kinase（ CK） at 20 and 40 min after reperfusion were determined. The myocardial tissues were stained with Triphenyltetrazolium chloride（TTC） and the percentage of myocardial infarction area was calculated. Results：HR,LVDP,± dp /dtmaxand CF of the I /R group were significantly decreased after reperfusion,LDH and CK levels were increased and the area of myocardial infraction was 58. 78%. TGRJ had improving effect on all above indexes. Conclusion：TGRJ has protective effet on the myocardial ischemic-reperfusion injury.

关 键 词：毛茛总苷 心肌缺血 再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]