亚硝基谷胱甘肽对冰冻血小板质量及功能的影响  

The impact of S-nitrosoglutathione on the quality and function of frozen platelets

作　　者：吴涛[1] 周俊[1] 晋晶[1] 刘景汉[2] 李卉[2] 周武[2] 王淑英[2] 

机构地区：[1]北京军区总医院输血科,北京100700 [2]解放军总医院输血科

出　　处：《中国输血杂志》2014年第10期1006-1009,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨亚硝基谷胱甘肽(GSNO)对冰冻血小板聚集、一氧化氮(NO)含量和膜糖蛋白分子的影响。方法实验分为3组(n=12):新鲜液态血小板组(对照1组)、冰冻血小板组(对照2组)、加有GSNO的冷冻血小板组(实验组)。用血小板聚集仪测定冷冻血小板聚集率,用硝酸酶还原法检测NO含量,用流式细胞仪测定冷冻血小板膜糖蛋白分子。结果血小板聚集率(%):对照2组与实验组分别为35.47±2.93 vs 24.43±3.07(P<0.05);NO浓度(μmol/L):对照1组与实验组分别为31.59±16.88 vs 45.64±6.31(P<0.05),对照2组与实验组分别为22.16±6.38 vs 45.64±6.31(P<0.05);膜糖蛋白分子CD42b(%):对照1组与实验组分别为90.46±6.65 vs 78.32±12.54(P<0.05),对照2组与实验组分别为76.94±15.66 vs 78.32±12.54(P>0.05);膜糖蛋白分子CD62P(%):对照1组与实验组分别为12.74±9.64 vs 26.34±9.97(P<0.05),对照2组与实验组分别为31.72±8.20 vs 26.34±9.97(P>0.05)。结论 GSNO增加了冰冻血小板NO的浓度,抑制血小板在贮存过程中的聚集,有望作为冰冻血小板的保护剂。Objective To investigate the impact of S-nitresoglutathione （GSNO） on frozen platelet aggregation, nitric oxide （NO） content and membrane glycoprotein molecules. Methods Samples were divided into three groups （n = 12） ： fresh liquid platelet group （control group 1 ） ,frozen platelet group （control group 2）, and a GSNO frozen platelet group （ex- perimental group）. Platelet aggregation was measured using platelet aggregation meter; NO content was measured by nitrate enzyme reduction assay; the percentage of platelet membrane glyeoprotein molecules was measured by flow cytometry. Results The platelet aggregation rate（ % ） of control group 2 and experimental group were 35.47±2.93 and 24.43 ±3.07（P 〈0. 05） ,respectively. The NO concentration （ μmol/L） of control group 1 and experimental group were 31.59± 16.88 and 45. 64± 6.31 （ P 〈 0. 05 ）, respectively; the NO coneentration of control group 2 and experimental group were 22.16 ± 6.38 and 45.64 ±6.31 （ P 〈 0. 05 ） ,respectively. The percentages of membrane glycoproteins CD42b in control group 1 and experimen- tal group were 90.46±6, 65 and 78.32 ± 12.54 （ P 〈 0. 05 ）, respectively; the percentages of CD42b in control group 2 and experimental group were 76.94 ± 15.66 and 78.32 ± 12.54 （P 〉 0. 05 ）, respectively. The percentages of membrane glycoproreins CD62P in control group 1 and experimental group were 12.74 ±9.64 and 26.34 ± 9.97 （P 〈 0. 05 ）, respectively;the percentages of CD62P in control group 2 and experimental group were 31.72 ± 8.20 and 26.34± 9.97 （ P 〉 0. 05 ）, respectively. Conclusion GSNO increased the NO concentration of frozem-plateleJs, inhibited platelet aggregation during storage, and was expected to protect cryopreserved platelets.

关 键 词：亚硝基谷胱甘肽 冰冻血小板 血小板聚集 一氧化氮 膜糖蛋白分子 

分 类 号：R457.12[医药卫生—治疗学] R331.143[医药卫生—临床医学]