附子水提组分对正常大鼠血压影响的“量-时-效”关系研究  

Experimental Studyon "Dose- time- efficacy" Relationshipof Blood Pressure Inducedby Aconite Water Extract

作　　者：李晓宇[1] 赵庆华[2] 吕莉莉[1] 王懿[3] 冯群[4] 严敏[5] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355 [3]山东省中医院,山东济南250011 [4]天津中医药大学,天津300193 [5]山东省疾病预防控制中心,山东济南250014

出　　处：《中国药物警戒》2014年第10期584-586,591,共4页Chinese Journal of Pharmacovigilance

基　　金：国家重点基础研究发展计划(973)中医基础理论专项资助项目课题2(2009CB522802)

摘　　要：目的研究药典规定剂量附子水提组分单次给药对正常大鼠血压影响的“量-时-效”关系。方法制备1．35、1．08、0．81、0．54、0．27g·kg-1（分别相当于药典规定附子用量15、12、9,6、3g）5个不同剂量组的附子水提组分，给予大鼠灌胃上述剂量的附子水提组分，分别于药前，药后15、30、60、90、120min，采用BP-100A全自动大小鼠无创血压测量系统观察不同剂量和时间下大鼠血压变似隋况，并计算附子水提组分对大鼠正常血压影响的ED50值。结果附子水提组分对大鼠收缩压“量-时-效”关系研究显示：5个剂量组大鼠收缩压在药后30min达峰，且水提组分1．35g·kg-1剂量组升高幅度最大，药后120min，5个剂量组的收缩压均基本回落到给药前水平；附子水提组分对大鼠舒张压“量-时-效”关系研究显示：水提组分对舒张压较给药前开始均有不同程度的升高；附子水提组分对大鼠正常血压影响的ED。值及其95％可信限为0．1474（2．3411～0．3482）g·kg-1。结论附子水提组分在药典规定剂量范围内使大鼠收缩压升高，并呈现一定的“量-时-效”关系，剂量愈高，升压作用愈大，作用时间愈长。Objective To study the influences of ＂dose-time-efficacy＂ relationship on normal rat blood pressure from a single administration of Aconite water extract in pharmacopoeia dose. Methods The 5 different Aconite water extract doses of 1.35, 1.08, 0.81, 0.54, 0.27 g·kg-1 （which correspond in the pharmacopoeia Aconite dose 15, 12, 9, 6, 3 g） were prepared, and rats were given Aconite water extract by gavage respectively. Changes of rat blood pressure according to different time and dose were observed in 15, 30, 60, 90, 120 min respectively by BP-100A automatic non-invasive blood pressure measurement system. And the EDs~ of Aconite water extract was tested. Results The ＂dose-time-efficacy＂ relationship showed that： compared with normal group, the changes of blood pressure in rat were increasing marks from 15 re_in to 120 min. The systolic pressure in five dose groups hit the peak in 30 rain and Aconite water extract high dose （1.35g·kg-1） is the largest. In 120 rain, the systolic pressure in five dose groups were basically back to the front of the gavage. The diastolic blood pressure of rats by Aconite water extract was increasing marks from 15 man to 120 rain to some extent. The ED50 and its 95% confidence limit of Aconite water extract were 0.1474 （2.3411--0.3482） g·kg-1. Conclusion Aconite water extract in the pharmacopoeia dose range elevates rats systolic blood pressure, and there is a certain relationship of ＂dose-time-efficacy＂： higher dose, greater pressor effects and longer action time.

关 键 词：附子 水提组分 血压 量-时-效 

分 类 号：R992[医药卫生—毒理学] R282[医药卫生—药学]