Cu(Ⅱ)对纤维蛋白原硝化影响的研究  

Research on the Influence of Cu(Ⅱ)on Fibrinogen Nitration

作　　者：史建龙[1] 罗云敬[1] 崔爽[1] 叶三仙[1] 鲁绯 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]北京市食品与酿酒产业产品质量监督检验一站,北京100075

出　　处：《化学与生物工程》2014年第10期20-23,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(20875006);北京市自然科学基金资助项目(2102005);北京市教委科技面上项目(KM201210005032)

摘　　要：选取机体Cu(Ⅱ)浓度,运用衰减全反射傅立叶变换红外光谱(ATR-FTIR)、SDS-PAGE电泳和冯克劳斯法,分析了Cu(Ⅱ)对过氧亚硝酸根介导的硝化反应中纤维蛋白原二级结构和凝聚活性的影响。结果显示:随着Cu(Ⅱ)浓度的增加,硝化纤维蛋白原中110kDa新增条带越来越清晰;二级结构中α-螺旋比例下降,β-折叠比例上升;凝聚活性逐渐降低;当Cu(Ⅱ)浓度为1.66mmol·L-1时,硝化纤维蛋白原中的α-螺旋比例由最初的32.29%减少到25.15%,β-折叠比例从35.66%升高到44.47%;冯克劳斯法检测硝化纤维蛋白原失去凝聚能力,但SDS-PAGE电泳表明其依然可以释放纤维蛋白单体。表明,Cu(Ⅱ)能显著影响硝化反应中纤维蛋白原的二级结构,抑制其凝聚活性,促进硝化损伤。The concentration of Cu（Ⅱ）was selected in biomimetic system to analyze the influence of Cu（Ⅱ） on secondary structure changes and aggregation activity of fibrinogen in nitration reaction by peroxynitrite with the aid of ATR-FTIR,SDS-PAGE and Von Clauss method.The results showed that with the increase of Cu（Ⅱ） concentration,a 110 kDa band became more and more clearer,theα-helix proportion declined whileβ-fold pro-portion increased in the secondary structure of nitration fibrinogen,and aggregation activity decreased gradual-ly.When Cu（Ⅱ）concentration increased to 1.66 mmol·L-1,theα-helix proportion decreased from initial 32.29% to 25.15%,whileβ-fold proportion increased from 35.66% to 44.47%.The nitration fibrinogen lost aggregation activity as detected by Von Clauss method,though SDS-PAGE showed that it still could release fi-brin monomer.The research demonstrated that Cu（Ⅱ）could significantly influence the fibrinogen secondary structure changes during nitration reaction,inhibit its aggregation activity and promote nitration inj ury.

关 键 词：过氧亚硝酸根 纤维蛋白原 红外光谱 冯克劳斯法 Cu(Ⅱ) 

分 类 号：O611.62[理学—无机化学] Q51[理学—化学]