小鼠肝脏大部分切除后改良再生模型的建立  

An Improved Model of Mouse Partial Hepatectomy for Study on Liver Regeneration

作　　者：施国明[1] 蔡加彬[1] 俞静娴 柯爱武[1] 董兆如[1] 张弛[1] 张鹏飞[1] 邱双健[1] 孙惠川[1] 周俭[1] 樊嘉[1] 

机构地区：[1]复旦大学附属中山医院肝外科,上海200032

出　　处：《中国临床医学》2014年第3期237-238,共2页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金资助项目(编号:81071741);上海市科委医学引导类项目(编号:114119a5000);教育部新教师基金(编号:20090071120025)

摘　　要：目的：探讨小鼠肝脏大部分切除后再生模型的改良制作方法。方法：将8～10周龄雄性C57BL/6小鼠腹腔麻醉后，用带针6-0 proline缝线环绕肝左外叶、肝中叶共同肝蒂，距肝蒂0．2 cm穿肝右中叶表面固定，结扎肝蒂，于结扎线远侧0．2 cm处切除小鼠肝脏左外叶、肝中叶（左、右）。观察小鼠术后成活率以及术后3d、7d、14d小鼠肝脏质量。结果：成功建立小鼠肝脏大部分切除模型，该模型切除小鼠肝左外叶、肝中叶（左、右），切除肝质量占肝总质量的66．7％，术后小鼠存活率100％。术后小鼠剩肝代偿性增生，术后第3天恢复至原肝总质量的58．2％，第7天恢复至原肝总质量的78．0％，术后第14天恢复至原肝总质量的93．2％。结论：本研究建立的改进型小鼠肝脏大部分切除后再生模型，具有简便、可操作性强、成功率高等优点。Objective：To establish an improved model of mouse partial hepatectomy for study on liver regeneration .Methods ：Healthy 8-10 week old male C57BL/6 mice were anesthetized by intraperitoneal injection .Then ,common hepatic pedicle of left lateral lobe of liver and middle lobe of liver was wrapped around with 6-0 proline suture .6-0 proline was fixed on the sur-face of right middle lobe of liver ,which was 0 .2 cm away from the hepatic pedicle .Hepatic pedicle was ligated and left lateral lobe and middle lobe of liver were resected 0 .2 cm away from ligature .The postoperative survival rate and the situation of liver weight vecovery at 3rd day ,7th day ,and 14th day after the operation were observed .Results：An improved mouse model of partial hepatectomy ,of which left lateral lobe of liver and middle lobe of liver were resected ,was established .The weight of removed liver accounted for 66 .7% of total liver and the survival rate of mice was 100% .After liver resection ,the compensa-tory regeneration of remaining liver achieved 58 .2% of the total liver weight at the 3rd day ,78 .0% at the 7th day ,and 93 .2%at the 14th day after the operation .Conclusions ：An improved mouse model of partial hepatectomy was successfully established for study on liver regeneration ,which has advantages such as simplicity ,operability and high success rate .

关 键 词：小鼠模型 肝大部分切除术 肝再生 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]