TWEAK对体外培养大鼠关节软骨细胞产生趋化因子的诱导作用  

Inducing Effect of TWEAK on Generating Chemokines in Rat Articular Chondrocytes Cultured in Vitro

作　　者：蔡国平[1] 熊敏[1] 张新潮[1] 刘德昌[1] 桂柯科[1] 

机构地区：[1]复旦大学附属金山医院骨科,上海201508

出　　处：《中国临床医学》2014年第3期247-250,共4页Chinese Journal of Clinical Medicine

基　　金：上海市卫生局科研项目(编号:20114258)

摘　　要：目的:探讨肿瘤坏死因子样弱凋亡诱导因子(tumor necrosis factor-like weak inducer of apoptosis,TWEAK)对体外培养的大鼠关节软骨细胞产生趋化因子的影响,初步了解TWEAK在OA发病机制中的作用。方法:体外培养大鼠关节软骨细胞,实验组细胞用250 ng/mL TWEAK诱导,对照组则不作此处理,24、48、72 h后检测两组细胞培养液中趋化因子白细胞介素-8(interleukin-8,IL-8)、单核细胞趋化蛋白-1(monocyte chemotactic protein 1,MCP-1)、受激活调节正常T细胞表达和分泌因子(regulated upon activation normal T cell expressed and secreted factor,RANTES)和巨噬细胞炎性蛋白-2(macrophage inflammatory protein 2,MIP-2)的含量。结果:TWEAK诱导24、48、72 h后,实验组大鼠关节软骨细胞上清液中RANTES含量均明显高于相应对照组(P<0.05),且RANTES的含量与TWEAK作用时间呈正相关(P<0.05);实验组IL-8和MCP-1的含量在TWEAK诱导48、72 h后均明显高于相应对照组(P<0.05),但与TWEAK作用时间无相关性(P>0.05);MIP-2的浓度在TWEAK诱导48、72 h后均明显低于相应对照组(均P<0.05),且其含量的降低与TWEAK作用时间呈正相关(P<0.05)。结论:TWEAK能诱导体外培养的大鼠关节软骨细胞产生趋化因子(IL-8、MCP-1、RANTES),以此参与OA的发生。Objective：To explore the effect of tumor necrosis factor-like weak inducer of apoptosis （TWEAK） on generating chemokines in rat articular chondrocytes cultured in vitro ,and to understand its role in the pathogenesis of OA .Methods ：Rat articular chondrocytes were cultured in vitro .The cells of experimental group were induced by 250 ng/mL TWEAK ,while the cells of the control group were not .Levels of chemokines interleukin-8（IL-8） ,monocyte chemotactic protein 1（MCP-1） ,regu-lated upon activation normal T cell expressed and secreted factor （RANTES） ,macrophage inflammatory protein 2（MIP-2） in the nutrient solution of the two groups were detected at 24 ,48 and 72 h .Results：After the induction of TWEAK ,the levels of RANTES in the supernatant of rat articular chondrocytes at 24 ,48 and 72 h were all significantly higher than those in the con-trol groups ,respectively （all P〈0 .05） ,and the contents of RANTES were positively correlated with the treatment time of TWEAK （P＆lt;0 .05） .The IL-8 and MCP-1 levels in the supernatant of rat articular chondrocytes at 48 and 72 h were signifi-cantly higher than those in the control groups （both P〈0 .05） ,but they had no correlations with the treatment time of TWEAK （P〉0 .05） .The MIP-2 levels of the rat articular chondrocytes supernatant at 48 and 72 h were significantly lower than those in the control groups （P〈0 .05） ,and the contents of MIP2 were positively correlated with the treatment time of TWEAK （P〈0 .05） .Conclusions ：TWEAK can induce rat chondrocytes in vitro to generate chemokines （IL-8 ,MCP-1 ,RAN-T ES ） ,so that it is involved in the occurance of OA .

关 键 词：关节细胞 肿瘤坏死因子样弱凋亡诱导因子 趋化因子 

分 类 号：R684.3[医药卫生—骨科学]