“细胞工厂”显微监测装置的光学设计  

Optical Design of“Cell Factory”Microscopic Monitoring Device

作　　者：李琦[1] 向阳[1] 谷俊达 董萌[1] 

机构地区：[1]长春理工大学,吉林长春130022

出　　处：《中国激光》2014年第10期267-272,共6页Chinese Journal of Lasers

摘　　要：基于目前国内对于细胞工厂（1～40层培养皿）监测观察方法的局限性,为了方便观察,节省时间,提高工作效率,设计出一款用于监测＂细胞工厂＂中培养细胞的长工作距离的视频显微物镜。设计利用卡塞格林反射系统,放大倍率为5×,工作距离85mm,数值孔径（NA）达到0.3,根据培养皿中的细胞大小,经过计算,运用ZEMAX软件进行模拟,配以维视图像生产的MV-200UC 1/3″电荷耦合器件（CCD）进行观察,接以视频显示器二次放大,总放大倍率M在200倍左右,系统分辨的最小距离在1.1μm左右,满足分辨培养皿细胞的要求。Due to the limitation of observation method in cell factory（1～40layer dishes）monitoring in China,this paper designs a long-working-distance video objective for observing the cultured cells in cell factory,to facilitate the observation,save time and improve efficiency.According to the size of cells,the high resolution video objective,with 5 times magnification and 85 mm working distance,is designed by applying ZEMAX stimulation and Cassegrain reflector system.Amplified again by the video display,the total magnification is about 200 times,the numerical aperture is up to 0.3 and the liner field object height y is 0.6 mm,equipped with MV-200 UC 1/3″charge couple device（CCD）produced by Microvision in observation.The minimum distance resolution of the system is about1.1μm,meeting the requirement of distinguishing petri dish cells.

关 键 词：光学设计 高分辨率 细胞工厂 长工作距 显微物镜 

分 类 号：TH742[机械工程—光学工程]