ox-LDL对破骨细胞增殖及分化的影响  

The effect of ox-LDL on the proliferation and differentiation of osteoclasts

作　　者：陈雪[1] 许娟[2] 亓文波[2] 

机构地区：[1]泰山医学院,山东泰安271016 [2]泰山医学院附属泰山医院,山东泰安271000

出　　处：《泰山医学院学报》2014年第7期581-583,共3页Journal of Taishan Medical College

摘　　要：目的检测ox-LDL对破骨细胞增殖及分化的影响。方法 CuSO4氧化法制备ox-LDL,硫代巴比妥法鉴定低密度脂蛋白氧化程度,将制备的ox-LDL配置成不同的浓度,对破骨细胞进行干预,MTT法测定其对破骨细胞增殖及分化的影响。结果不同浓度的ox-LDL对破骨细胞的增殖及分化的影响有差别:ox-LDL浓度分别为100mg/L,150 mg/L,200 mg/L时,破骨细胞抑制率为0.65±0.732,0.48±0.068,0.31±0.039,都较对照组破骨细胞的1.28±0.147低,P<0.05。而ox-LDL浓度为25 mg/L及50mg/L时,破骨细胞抑制率为1.134±0.120,1.189±0.112,P>0.05。结论成功制备了氧化性低密度脂蛋白及诱导形成破骨细胞,一定浓度的ox-LDL可促进破骨细胞的增殖及分化。Objective：To explore the effect of ox-LDL on the proliferation and differentiation of osteoclasts. Methods：ox-LDL was prepareed by copper ion oxidation. Degree of ox-LDL was identified by thiobarbituric. Prepared ox-LDL was configured into different concentrations and added to osteoclasts. The effects on osteoclast proliferation and differentiation were assayed by the method of MTT. Results：The degree of osteoclast proliferation and differentiation differed significantly among the groups of different concentrations of ox-LDL. When ox-LDL concentration was 100mg / L,150mg/ L and 200 mg/ L,osteoclast inhibition rate was 0. 65 ± 0. 732,0. 48 ± 0. 068 and 0. 31 ± 0. 039 respectively,which was significantly lower than those of the control group（1. 28 ± 0. 147,P ﹤ 0. 05）. The ox-LDL concentration of 25 mg/ L and 50mg/ L,the osteoclast inhibition rate was 1. 134 ± 0. 120 and 1. 189 ± 0. 112 respectively（P ﹥ 0. 05）. Conclusion：A certain con-centration of ox-LDL can promote proliferation and differentiation of osteoclasts.

关 键 词：氧化性低密度脂蛋白 破骨细胞 增殖及分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]