桑白皮水煎液及各化学拆分组分利尿作用研究  

Study on Diuretic Effect of Mori Cortex Aqueous Extracts and Chemical Split Fractions

作　　者：郑晓珂[1] 李玲玲[1] 曾梦楠[1] 王绅[1] 黄岩杰[2] 李静[2] 赫金丽 曹彦刚 冯卫生[1] 

机构地区：[1]河南中医学院药学院,郑州450046 [2]河南中医学院第一附属医院,郑州450000

出　　处：《世界科学技术-中医药现代化》2014年第9期1946-1950,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重点基础研究发展计划"973计划"项目(2013CB531802):宣泻利水中药的药性研究;负责人:郑晓珂

摘　　要：目的:探讨桑白皮的利尿作用及发挥利尿作用的有效部位。方法:采用大鼠代谢笼法,首先观察桑白皮水煎液的利尿作用,连续给药5天,筛选出发挥利尿作用的最佳剂量,并用苦味酸法检测给药5天后血清和尿液中肌酐的含量;进而研究桑白皮各化学拆分组分的利尿作用,确定桑白皮发挥利尿作用的有效部位。结果:桑白皮水煎液给药第3—5天后,与对照组相比,大鼠尿量明显增加(P<0.05或P<0.01),且中剂量利尿效果最为显著(P<0.01);桑白皮水煎液对血肌酐、尿肌酐含量没有显著影响;各化学拆分组分利尿结果显示,与对照组相比,30%乙醇组分和脂肪油组分利尿作用最好(P<0.01)。结论:桑白皮水煎液具有明显的利尿作用,且发挥利尿作用的物质基础主要集中在30%乙醇组分和脂肪油组分。This study was aimed to investigate the diuretic effect ofMori Cortex and to identify effective fractions inM. Cortex. Metabolic cages were used to firstly observe the diuretic effect ofM. Cortex aqueous extracts on rats. Medications were continuously given for 5 days to screen for the best dosage of diuretic effect. Picric acid assays were used to detect creatinine levels in serum and urine after 5-day medication. Then, the diuretic effect of each chemical split fraction inM. Cortex was studied in order to indentify the effective parts. The results showed that after administration ofM. Cortex aqueous extracts for 3 to 5 days, compared with the control group, there was a significant diuretic effect on rats （P〈0.05 orP〈0.01）. And the medium-dose ofM. Cortex aqueous extracts showed the best effect （P〈0.01）. However,M. Cortex aqueous extracts had no significant effect on creatinine levels in serum and urine. Assays of diuretic effect of chemical split fractions ofM.Cortex indicated that compared with the control group, 30% ethanol fraction and fatty oil fraction had the best diuretic effect （P〈0.01）. It was concluded thatM.Cortex aqueous extracts had a significant diuretic effect. And the chemical fractions contributed mostly to this effect were mainly existed in the 30% ethanol fraction and fatty oil fraction.

关 键 词：桑白皮 化学拆分组分 利尿作用 药理研究 

分 类 号：R285.5[医药卫生—中药学]