Is the mission to identify all the human proteins achievable? —Commenting on the human proteome draft maps  

作　　者：CHANG ZengYi GU LiangCai 

机构地区：[1]Center for Protein Science, State Key Laboratory of Protein and Plant Gene Studies, School of Life Sciences, Center for History and Philosophy of Science, Peking University [2]Department of Genetics, Harvard Medical School

出　　处：《Science China(Life Sciences)》2014年第10期1039-1040,共2页中国科学（生命科学英文版）

摘　　要：Unveiling the structure and function of all the proteins a human body produces is undoubtedly a key task for us to better understand ourselves. Although a mission difficult to achieve, this apparently becomes more feasible upon the determination of the DNA sequence of the whole human genome, which was predicted to encode a total of 20,000-25,000 proteins [1]. Recent efforts of direct （de novo） protein isolation and peptide sequencing, mainly using liquid chromatography-tandem mass spectrometry （LC-MS/ MS） and in-depth bioinformatics analyses, led to the apparent identification of over 17,000 proteins from large sets of human tissues, cell lines and body fluids [2,3].Unveiling the structure and function of all the proteins a human body produces is undoubtedly a key task for us to better understand ourselves.Although a mission difficult to achieve,this apparently becomes more feasible upon the determination of the DNA sequence of the whole human genome,which was predicted to encode a total of20,000–25,000 proteins[1].Recent efforts of direct(de no-

关 键 词：人类蛋白质组 DNA序列 LC-MS 地图 人类基因组 串联质谱法 蛋白分离 液相色谱 

分 类 号：Q51[生物学—生物化学]