“勿忘我”丛生芽诱导及植株再生  

Study on Cluster Bud Induction and Plant Regeneration of Myosotis sylvatica

作　　者：王淑敏 高永闯 李晓云 

机构地区：[1]廊坊师院生命科学学院,河北廊坊065000

出　　处：《安徽农业科学》2014年第31期10842-10844,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究"勿忘我"丛生芽诱导及植株再生。[方法]以勿忘我为试材,取其茎尖、叶片和下胚轴为外植体,进行丛生芽诱导试验。[结果]用茎尖作外植体产生丛生芽数量最多;以MS+6-BA 1.0 mg/L+NAA0.2 mg/L诱导成苗率最高,芽苗健壮;生根阶段以1/2MS+IBA0.2mg/L生根效果最好,生根率可达100%。[结论]研究勿忘我丛生芽诱导及植株再生具有一定的实践意义和应用价值。[ Objective ] The aim was to study cluster bud induction and plant regeneration of Myosotis sylvatica. [ Method ] Using Myosotis sylvatica as test materials, cluster bud induction was carried out taking its stem tip, leaf and hypocotyl as explant. [ Result ] Cluster bud induction number was the largest using stem tip as explant. MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.2 mg/L induction rate was highest, and bud seedling was strong; 1/2 ms ＋ IBA0.2 mg/L rooting effect was best in rooting stage, and rooting rate could reach 100%. [ Conclusion] Studying cluster bud induction and plant regeneration of Myosotis sylvatica had certain practical.

关 键 词：勿忘我 丛生芽 再生植株 

分 类 号：S188[农业科学—农业基础科学]