UPLC法测定五味温通除痹胶囊中黄芩苷的含量  

Content determination of baicalin in Wuwei Wentong Chubi capsule by UPLC

作　　者：姜辉[1] 刘健[1] 孟楣[1] 桂洁[1] 

机构地区：[1]安徽中医药大学第一附属医院,国家中医药管理局中药制剂三级实验室,现代中医内科应用基础与开发研究实验室,安徽合肥230031

出　　处：《山西中医学院学报》2014年第3期37-38,41,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家中医药管理局重点学科-中医痹病学科开放基金(2011bbxk009);安徽中医药大学临床科研基金(2012LC1-023B);国家中医药重点学科临床中药学建设项目{国中医药人教发[2012]32号}

摘　　要：目的:建立五味温通除痹胶囊中黄芩苷的含量测定方法。方法:采用超高液相色谱法,以Acquity UPLC BEH C18柱(100 mm×2.1 mm,1.7μm)为色谱柱,乙腈-0.1%磷酸(25∶75)为流动相;流速:0.25 mL/min;柱温:30℃;检测波长280 nm。结果:黄芩苷在0.095 5 mg^0.955 mg范围内呈良好的线性关系,平均回收率为99.21%,RSD=1.16%。结论:该法准确可靠、重复性好,可用于该制剂中黄芩苷的含量测定。Objective：To establish a method for content determination of baicalin in Wuwei Wentong Chubi capsule by UPLC. Methods：UPLC method was performed on the acquity UPLC BEH C18（100 mm×2.1 mm,1.7μm）column with ace-tonitrile-0.1%phosphoric acid（25∶75）as mobile phase and the flow rate was 0.25 mL/min. Detection wavelength was set at 280 nm and the column temperature was 30 ℃. Results：The linear ranger of baicalin was 0.095 5 μg^0.955 0 μg. Aver-age recovery rate was 99.21% and RSD was 1.16%. Conclusions：The method is accurate,reliable and practical,and can be used for the quality control of Wuwei Wentong Chubi capsule.

关 键 词：五味温通除痹胶囊 UPLC 黄芩苷 含量测定 

分 类 号：R285.5[医药卫生—中药学]