Diagnostic and monitoring values of circulating AFPmRNA and HS-GGT for hepatocellular carcinoma  

循环血AFP-mRNA与HS-GGT检测对肝癌的诊断与监测价值(英文)

作　　者：Li Wang Min Yao Yao Yao Liuhong Pan Shaolin Lu Dengfu Yao 

机构地区：[1]Research Center of Clinical Medicine, Affiliated Hospital of Nantong University [2]Medical School of Nantong University [3]The Hospital of Nantong Maternal and Child Care Service [4]Center of Oncology, Affiliated Hospital of Nantong University

出　　处：《The Chinese-German Journal of Clinical Oncology》2014年第10期457-463,共7页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from the National Natural Science Foundation of China(No.81200634);the Projects of Jiangsu Medical Science(No.WSW-011,HK201102,BL2012053);the Qinglan Project of Jiangsu higher Education;he International S&T Cooperation Program of China(No.2013DFA32150)

摘　　要：Objective: Early finding of hepatocellular carcinoma(HCC) and monitoring of its metastasis are of the utmost importance. The objectives of this study were to investigate the values of circulating hepatoma-specific gamma-glutamyl transferase(HS-GGT) fraction and α-fetoprotein mRNA(AFP-mRNA) from peripheral blood mononuclear cells(PBMCs) in diagnosis or monitoring metastasis of HCC. Methods: Total RNA was extracted from hepatomas or PBMCs of patients, synthesized to AFP-cDNA through random primers and reverse transcriptase, amplified by using a nested PCR assay, and confirmed by sequencing. Simultaneity, the HS-GGT activities were quantitatively determined in sera of patients. The comprehensive assessments of two markers were investigated in HCC patients. Results: The amplified fragments of AFP-mRNA were identical to original designed ones(159 bp) and confirmed by sequencing. The lowest sensitivity was 2 ng/L of total RNA. The incidences of AFP-mRNA and HS-GGT were 60.4%(113 of 187) and 84.5%(158 of 187) in the HCC group, and their significantly higher(P < 0.01) than that in any of other groups. The high frequencies of HS-GGT and AFP-mRNA were 78.2% and 56.4% in the HCC patients with AFP < 50 ng/mL. All positive of AFP-mRNA was found in HCC patients with extrahepatic metastasis. No significant correlation was found between tumor size and AFP-mRNA or HS-GGT. The total incidence of combined AFP-mRNA and HS-GGT detection was 93.6 % for HCC diagnosis. Conclusion: HS-GGT and AFP-mRNA are specific markers for HCC diagnosis or differentiation, especially the former for early finding and the later for monitoring metastasis of HCC.

关 键 词：hepatocellular carcinoma（HCC） AFP-MRNA hepatoma-specific gamma-glutamyl transferase diagnosis metastasis 

分 类 号：R735.7[医药卫生—肿瘤]