乙型流感病毒在不同胚龄鸡胚中适宜孵育条件的研究  

Studying of Appropriate Incubation Conditions of Influenza B Virus in Different Embryonic Chicken Embryos

作　　者：李孙华[1] 靳亮[2] 

机构地区：[1]天津天士力研究院,天津300410 [2]江西省科学院微生物研究所,南昌330029

出　　处：《江西科学》2014年第5期636-639,共4页Jiangxi Science

基　　金：国家自然科学基金项目(31260031)

摘　　要：为了找出乙型流感病毒毒株在不同胚龄鸡胚中适宜孵育条件,提升血凝滴度,从而为产业化生产人用3价流感疫苗提高产能,主要采用血凝法检定收获尿囊液,分析试验血凝滴度数据,得出结论:乙型流感病毒毒株接种于10 d胚龄鸡胚,其孵育温度为35℃,孵育时间72 h最理想;孵育温度和时间对乙型流感病毒的血凝滴度有影响,同时鸡胚胚龄与其的血凝滴度和收获尿囊液量也有明显的关联。The appropriate incubation conditions of influenza B virus in different embryonic chicken were studied by hemagglutination assay of allantoic fluid analysis test and hemagglutination titers.The results showed that the best incubation temperature was 35 ℃ and the best incubation time was72 h when influenza B virus strains were inoculated into 10-days-old chicken embryos. Incubation temperature and time contacted with the hemagglutination titers of influenza B virus. Furthermore,the embryo age was significantly related to the hemagglutination titer and harvest allantoic fluid.

关 键 词：乙型流感病毒 鸡胚 孵育 血凝滴度 

分 类 号：S852.65[农业科学—基础兽医学]