一株稻纵卷叶螟颗粒体病毒的系统发育分析和流行病学调查  

Phylogenetic analysis and epidemiologic investigation of a Cnaphalocrocis medinalis granulovirus strain

作　　者：张珊[1] 贾茜雯 孙士锋 庞义[1] 陈其津[1] 杨凯[1] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室,广州510275 [2]华南师范大学附属中学,广州510630 [3]广东海纳农业有限公司,广东惠州516005

出　　处：《环境昆虫学报》2014年第5期756-762,共7页Journal of Environmental Entomology

基　　金：有害生物控制与资源利用国家重点实验室开放课题(SKLBC13KF01);广州"科技之星"青少年科技创新能力特训营培育活动

摘　　要：稻纵卷叶螟颗粒体病毒(Cnaphalocrocis medinalis granulovirus,CnmeGV)于1979年首次从我国水稻主要害虫稻纵卷叶螟Cnaphalocrocis medinalis Guenée幼虫中分离,能够在宿主种群内传播,具备发展为生物杀虫制剂的潜力。本研究利用从田间采集的稻纵卷叶螟幼虫,在实验室成功增殖了CnmeGV。编码杆状病毒RNA聚合酶两个亚基的基因lef-8、lef-9和包涵体基因granulin在杆状病毒系统演化过程中非常保守。利用PCR扩增得到CnmeGV的上述三个基因,并将其核苷酸序列融合后与GenBank中相应病毒序列进行系统发育分析,UPGMA演化树分析结果表明CnmeGV是未被GenBank收录的病毒。流行病学初步调查结果表明,仅在采集自广东省江门市恩平县的稻纵卷叶螟体内发现了CnmeGV,而其他采集地点,包括广州市岑村、惠州市万芦村、惠州市新岗村和湖北省潜江市均未发现。江门市恩平县是CnmeGV的首次发现地,时隔30多年,仍然存在因感染CnmeGV而死亡的稻纵卷叶螟幼虫,表明该病毒具有田间控制目标害虫的持效性能。Cnaphalocrocis medinalis granulovirus （CnmeGV） was first isolated in 1979 from C. medinalis larvae that is one of the main migratory pests of rice in China. CnmeGV epidemic disease is often observed in C. medinalis population, and thus the virus has a potential to be used as a biological pesticide. Here we report that CnmeGV was propagated successfully in the laboratory by using C. medinalis larvae collected from field. In baculoviruses, lef - 8, lef - 9 and granulin are highly conserved genes during evolution, lef- 8 and lef- 9 encode two subunits of the baculovirus RNA polymerase, and granulin encodes the major matrix protein of granulovirus occlusion bodies. These genes were PCR -amplified from the CnmeGV genomes and the nucleotide sequences of the three genes were concatenated for phylogenetic analysis. The resulting UPGMA distance tree suggested that CnmeGV was a distinct species and was not found in the GenBank database. Epidenfiologic studies showed that CnmeGV was detected in the population of C. medinalis larvae from Jiangmen Enping, but not from Huizhou Wanlu and Xingang , and Cencun, Guangzhou, in Guangdong Province as well as Qianjiang, Hubei Province. Interestingly, Enping is the place where CnmeGV was first isolated. The fact that CnmeGV has existed stably in the C. medinalis population in this area for more than 30 years suggests that CnmeGV is a potential effective pathogen to control the target pest in field.

关 键 词：稻纵卷叶螟颗粒体病毒 系统发育分析 流行病学 

分 类 号：Q965.8[生物学—昆虫学] S435[农业科学—农业昆虫与害虫防治]