25个油茶优良无性系的遗传分析与分子鉴别  

Genetic analyses and molecular identification of 25 superior clones of Camellia oleifera

作　　者：孙佩光[1,2] 奚如春[2,3] 李俊成[2,3] 欧阳昆唏[2,3] 钟燕梅[2,3] 陈晓阳[2,3] 

机构地区：[1]中国热带农业科学院海口实验站/海南省香蕉遗传改良重点实验室,海南海口570102 [2]华南农业大学亚热带农业生物资源保护与利用国家重点实验室/广东省森林植物种质创新与利用重点实验室,广东广州510642 [3]华南农业大学林学院,广东广州510642

出　　处：《华南农业大学学报》2014年第6期83-88,共6页Journal of South China Agricultural University

基　　金：广东省自然科学基金团队项目(9351064201000002);广东省林业科技创新专项(2012KJCX011-02)

摘　　要：【目的】为油茶种质资源的合理利用奠定理论基础，为油茶优良品种资源保护提供技术支持．【方法】采用SRAP分子标记对来源于江西省的25个油茶优良无性系进行遗传分析与分子鉴别．【结果和结论】11对SRAP引物组合共扩增出347个位点，其中多态性位点332个，多态性位点占比为95.68％．聚类分析结果表明，25个油茶优良无性系的遗传距离介于0.2556～0.7384，平均遗传距离为0.5999，表明这些油茶无性系的地理来源相近．在遗传距离为0.5280处，可以将25个油茶优良无性系分为5组．利用筛选出的引物构建了25个油茶优良无性系的DNA指纹图谱，每一对引物构建的指纹图谱均可以用于25个优良无性系的分子鉴别．[Objective]This study aimed to lay a theoretical foundation for rational use of Camellia oleifera germplasm resource and provide technical support for the conservation of elite C.oleifera varieties.[Method]SRAP molecular markers were used to assess the genetic diversity and molecular identification of 25 superior clones of C.oleifera from Jiangxi Province .[Result and conclusion]Three hundred ard forty seven sites were amplified by 11 primer combinations of SRAP , among which 332 were polymorphic sites . The percentage of polymorphic sites was 95.68%.The result of cluster analysis indicated that the genetic distance of the 25 superior clones ranged from 0.255 6 to 0.738 4, and the average genetic distance was 0.599 9 , indicating that these cultivars had similar geographical origins .The 25 superior clones were di-vided into five groups by cluster analysis according to genetic distance 0.528 0 .DNA fingerprints of the 25 superior clones were constructed using the screened primer combinations .The fingerprint constructedby each primer combination can be used for molecular identification of the 25 superior clones .

关 键 词：油茶 优良无性系 SRAP 遗传分析 分子鉴别 

分 类 号：S727.32[农业科学—林木遗传育种]