HPLC法测定银杏叶缓释微丸胶囊中山奈素、异鼠李素、槲皮素的含量  

Determination of the Content of Haempferol, Isorhamnetin and Quercetin in Folium Ginkgo Pellets Capsules by HPLC

作　　者：江东波[1] 马晓鹂[1] 李朝晖[1] 蔡伟明[1] 官堂明[1] 

机构地区：[1]广东医学院附属医院药学部,广东湛江524001

出　　处：《中国医药指南》2014年第29期12-13,共2页Guide of China Medicine

摘　　要：目的：建立以高效液相色谱法测定银杏叶缓释微丸胶囊中山奈素、异鼠李素、槲皮素含量的方法。方法以C18为色谱柱，流动相为甲醇-0.4%磷酸溶液（45∶55），检测波长为360nm，流速为1mL/min，柱温为30℃，进样量是10μL。结果槲皮素在0.1505～0.9030μg（r＝0.9999）浓度范围内有良好的线性关系，平均回收率为99.86%，RSD=0.4%；山奈素在0.1450~0.8940μg（r＝0.9998）浓度范围内有良好的线性关系，平均回收率为99.79%，RSD=0.5%；异鼠李素在0.0995~0.5970μg（r＝0.9998）浓度范围内有良好的线性关系，平均回收率为99.80%， RSD=0.3%。结论本方法简便、准确、专属性强，可用于该制剂的质量控制。Objective To establish a method for the determination of haempferol, isorhamnetin and quercetin in folium ginkgo pellets capsules Methods HPLC was performed on a C18 column using mathanol-0.4%H3PO4 （45∶55） as the mobile phase at a lfow rate of 1 mL/min. The detection wavelength was at 360 nm. The column temperature was 30℃and the sample size was 10μL. Results The linear range of quercetin was 0.1505-0.9030μg （r=0.9999）, the average recovery was 99.86%（RSD=0.4%）;the linear range of haempferol was 0.1450-0.8940μg （r=0.9998）, the average recovery was 99.79%（RSD=0.5%）;the linear range of isorhamnetin was 0.0995-0.5970μg （r=0.9998）, the average recovery was 99.80%（RSD=0.3%）. Conclusions The method is simple, accurate, and special and can be used for the quality control of folium ginkgo pellets capsules.

关 键 词：银杏叶缓释微丸胶囊 山奈素 异鼠李素 槲皮素 高效液相色谱法 

分 类 号：R9[医药卫生—药学]