结核γ-干扰素酶联免疫斑点检测在结核性心包积液诊断中的应用  

Utility of interferon-gamma enzyme-linked immunosorbent spot assay in the diagnosis of tuberculous pericardial effusion

作　　者：孟娟[1] 廖明凤[2] 孙秀才[1] 施霞[1] 张明霞[2] 陈心春[2] 

机构地区：[1]深圳市第三人民医院心血管内科,广东深圳518112 [2]深圳市第三人民医院 深圳市感染免疫重点实验室,广东深圳518112

出　　处：《岭南心血管病杂志》2014年第5期632-635,共4页South China Journal of Cardiovascular Diseases

基　　金：国家自然科学基金面上项目(项目编号:81241065);深圳市科技计划项目(项目编号:201202064)

摘　　要：目的探讨结核菌特异性γ-干扰素(interferon-gamma,IFN-γ)酶联免疫斑点(enzyme-linked immunosorbent spot,ELISPOT)检测对结核性心包积液的诊断价值。方法采用结核菌特异性IFN-γELISPOT技术同时检测20例结核性心包积液患者(TP组)和14例非结核性心包积液患者(Non-TP组)外周血单核细胞(peripheral blood mononuclear cells,PBMC)及心包积液单核细胞(pleural effusion mononuclear cells,PEMC)中结核菌特异性IFN-γ水平。结果 TP组PBMC和PEMC结核菌特异性IFN-γ水平均显著高于Non-TP组,差异有统计学意义(P<0.01,P<0.01)。TP组心包积液中结核菌特异性IFN-γ水平显著高于外周血IFN-γ水平,差异有统计学意义(P<0.01,P<0.01)。PBMC ELISPOT检测结核性心包积液的敏感性和特异性分别为80.0%和85.7%;而PEMC ELISPOT敏感性和特异性为90.0%和85.7%。结论结核菌特异性IFN-γELISPOT技术对结核性心包积液诊断和鉴别诊断具有很好的辅助价值。Objectives To observe the clinical utility of the mycobacterium tuberculosis antigen-specific interferon-gamma （IFN-γ） enzyme-linked immunosorbent spot （ELISPOT） assay in the diagnosis of tuberculous pericardial effusion.Methods IFN-γELISPOT assay was used to determine the mycobacterium tuberculosis antigen-specific IFN-γ productions of peripheral blood mononuclear cells （PBMCs） and pleural effusion mononuclear cells （PEMCs） in 20 patients with tuberculous pericardial effusion （TP group） and 14 patients with non-tuberculous pericardial effusion （Non-TP group）.Results The productions of antigen-specific IFN-γin PBMCs and in PEMCs of tuberculous pericardial effusion patients were significantly higher than those of non-tuberculous pericardial effusion patients （P〈0.01,P〈0.01）.The production of antigen-specific IFN-γin PEMCs was much more higher than that in PBMCs of tuberculous pericardial effusion patients （P〈0.01,P〈0.01）.The sensitivity and specificity of ELISPOT assay in PBMCs and PEMCs were 80.0%,85.7% and 90.0%,85.7% respectively.Conclusions Mycobacterium tuberculosis antigen-specific IFN-γ ELISPOT assay is useful in improving the diagnosis of tuberculous pericardial effusion.

关 键 词：结核性心包积液 诊断 酶联免疫斑点技术 

分 类 号：R542.12[医药卫生—心血管疾病]