红树林沉积物中产氢微生物的分离鉴定和性能分析  

Isolation and Characterization of a Morganella morganii Strain with Hydrogen-producing Activities from the Mangrove Sediments

作　　者：朱大玲[1] 杨振芳[1] 潘光华[1] 

机构地区：[1]天津市海洋化学与资源重点实验室,天津科技大学海洋科学与工程学院

出　　处：《天津科技大学学报》2014年第5期29-34,共6页Journal of Tianjin University of Science & Technology

基　　金：天津市自然科学基金资助项目(12JCQNJC04300);天津市海洋化学与资源重点实验室基金资助项目(201203)

摘　　要：采用厌氧富集三层平板和螺口试管培养法,根据产气特征从红树林沉积物中筛选分离海水条件下的高效产氢菌株,获得1株兼性厌氧具有较高产氢能力的菌株BH-16.通过形态观察、Biolog鉴定和16S rDNA序列分析,该菌株鉴定为摩氏摩根氏菌BH-16.实验表明:在厌氧培养条件下,菌株BH-16的最适起始pH、NaCl质量浓度和培养温度分别为8.0、0.01 g/mL、37,℃.通过间歇产气实验和气相色谱分析对菌株的产氢能力进行分析,结果表明:菌株BH-16大量产氢发生在细胞指数生长后期和细胞生长平稳期;在海水培养条件下,在起始葡萄糖质量浓度为20 g/L、起始pH为7.2时,菌株的总产氢量和平均产氢速率分别为1,120 mL/L和93.3 mL/(L·h).According to the property of gas production,highly hydrogen-producing bacterial strain BH-16 was screened in the culture condition of marine water from the mangrove sediment. The screening and isolation of the strain were realized with the methods of anaerobic enrichment,three-layer plate and the culture in the tube with nut. Strain BH-16 was identified and designated as Morganella morganii with light microscopic examination,Biolog test and 16S rDNA sequence analysis. Experiments show that in the anaerobic culture,the optimum initial pH value,NaCl concentration and temperature for cell growth were 8.0,0.01 g/mL and 37,℃,respectively. During fermentation,hydrogen started to evolve when cell growth en-tered the middle-exponential phase and was mainly produced in the late-exponential phase and the stationary phase. The total hydrogen-production yield（1,120 mL/L）and the overall hydrogen production rate（93.3 mL/（L·h））were obtained at an initial glucose concentration of 20 g/L and an initial pH value of 7.2 in marine culture conditions.

关 键 词：红树林沉积物 摩氏摩根氏菌 分离鉴定 发酵产氢 

分 类 号：Q935[生物学—微生物学]