醋柳黄酮通过钙调神经磷酸酶途径抑制心肌肥厚的研究  

Study on the inhibition of total flavones of hippophae rhamnoides on cardiac hypertrophy by calcineurin pathway

作　　者：汪接根[1] 余琴[2] 刘应才[2] 

机构地区：[1]四川省兴文县中医医院,四川兴文644400 [2]泸州医学院附属医院,四川泸州646000

出　　处：《现代中西医结合杂志》2014年第32期3544-3547,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨醋柳黄酮是否通过钙调神经磷酸酶途径抑制心肌肥厚。方法通过腹主动脉缩窄法构建高血压心肌肥厚大鼠模型,随机分成4组(n=8):对照组(Control组,饮用水10 mL/(kg·d)),卡托普利组(CP组,100 mg/(kg·d)),醋柳黄酮组(TFH组,TFH 30mg/(kg·d)),联合组(Com组,CP 60 mg/(kg·d)+TFH 20 mg/(kg·d)),另8只大鼠作为假手术组(Sham组,饮用水10 mL/(kg·d)),连续灌胃干预6周。采用套尾法测量术前,术后1周、7周大鼠尾动脉血压。测量术后7周各组大鼠左心室质量/体质量比、钙调神经磷酸酶(CaN)活性及CaN-mRNA表达。结果腹主动脉缩窄术后的大鼠血压、左心室质量/体质量比、CaN活性及CaN-mRNA表达均明显升高(P均<0.05),TFH能够明显抑制这些变化(P均<0.05),并且TFH和CP有协同作用。结论醋柳黄酮具有显著降压功效,能抑制心肌肥厚的发生,其机制可能与抑制钙调神经磷酸酶的表达及其活性相关,醋柳黄酮与卡托普利具有协同作用。Objective It is to explore the inhibition of total flavones of hippophae rhamnoides（ TFH） on cardiac hypertrophy whether through calcineurin signal pathway. Methods 32 rats were partially banded abdominal aortic artery to establish the hypertensive models and were divided into four groups randomly（ n = 8） ： control group（ potable water 10 mL /（ kg·d））,captoprill（ CP group,100 mg /（ kg·d））; TFH group（ 30 mg /（ kg·d））,combination group（ Com group,CP 60 mg /（ kg·d） ＋TFH 20 mg /（ kg·d））. They were gastric perfused once a day for 6 weeks. Other 8 rats with sham-operation were setted as sham group（ potable water 10 mL /（ kg·d） for 6 weeks）. Blood pressures of tail artery were measured by tail-cuff method in the periods of pre-operation,after-operation for 1st and 7th week respectively. The left ventricle weight /body weight（ g /100g） ratio,calcineurin（ CaN） activity and the mRNA levels of CaN were measured 7 weeks after operation. Results The blood pressure,left ventricle weight /body weight（ g /100 g） ratio,CaN activity and the mRNA levels of CaN of the hypertensive rats increased obviously（ P〈 0. 05）. But the changes could be inhibited by TFH,and TFH and CP had a synergistic effect. Conclusion TFH has significant anti-hypertension efficiency and could prevent cardiac hypertrophy,and the mechanism may be related with the inhibition of the expression of the CaN signal pathway and its activity,and the combination of both drugs has synergistic work.

关 键 词：醋柳黄酮 心肌肥厚 钙调神经磷酸酶 

分 类 号：R541[医药卫生—心血管疾病]