血管性认知障碍大鼠学习记忆、血清EPCs、NO、VEGF变化及电针的干预作用  

Electroacupuncture on learning memory and EPCs,NO,VEGF of vascular dementia rats

作　　者：钱善军[1] 陈幸生[2] 周婷[2] 程红亮[2] 张闻东[2] 李飞[2] 蔡荣林[3] 吴生兵[3] 陈国庆[3] 王储蓄 何俊良[3] 

机构地区：[1]安徽医学高等专科学校,合肥230601 [2]安徽中医药大学第二附属医院,合肥230061 [3]安徽中医药大学,合肥230038

出　　处：《长春中医药大学学报》2014年第5期770-772,共3页Journal of Changchun University of Chinese Medicine

基　　金：安徽省自然科学基金项目(1208085MH126)

摘　　要：目的观察电针对血管性痴呆(VD)大鼠学习记忆、内皮祖细胞(EPCs)数量、氧化亚氮(NO)及血管内皮生长因子(VEGF)的影响。方法四血管阻断制作VD模型大鼠,水迷宫检测大鼠学习记忆能力、流式细胞术检测外周血内皮祖细胞(EPCs)数量、ELISA放射免疫法检测VEGF含量、硝酸还原酶法检测NO并与茴拉西坦对照。结果与空白对照组比较,电针能够改善血管性认知障碍大鼠的学习记忆能力,提高外周血血管内皮祖细胞的数量,升高血清NO和VEGF水平。结论电针可通过促进内皮祖细胞的增殖、分化,上调血管生长因子NO和VEGF表达,从而促进脑微血管新生,恢复神经功能,提高学习记忆能力。Objective To observe the effect of electroacupuncture on learning memory ability and the changes of EPC, NO, VEGF in plasma of vascular dementia （VD） rats. Methods After induced by 4-vessle occlusion, morris water maze tests were conducted for evaluating the rats learning-memory ability, the contents of EPCs in plasma were measured by flow cytometry, VEGF was determined by ELISA, NO by Nitrate reductase respectively, and compared to aniracetam. Results Compared to the control group, electroacupuncture could elevate the learning and memory ability, increased the content of EPCs, NO and VEGF. Conclusion Electroacupuncture can elevate the learning and memory ability by Promoting angiogenesis.

关 键 词：血管性认知障碍 针灸疗法 内皮祖细胞 血管新生 大鼠 

分 类 号：R285.5[医药卫生—中药学]