参红补血颗粒对乙酰苯肼/环磷酰胺血虚证模型动物免疫调节相关因子的影响  

Effects of Shenhong Buxue Granule on the related cytokines of immunoregulation of model animal with phenylhydrazine /cyclophosphamide type of blood deficiency

作　　者：张文卓[1] 杨海淼[1] 张硕[1] 常影[1] 崔云晶 仇志凯[1] 段青[2] 黄晓巍[1] 

机构地区：[1]长春中医药大学,长春130117 [2]中国中医科学院,北京100700

出　　处：《长春中医药大学学报》2014年第5期776-778,共3页Journal of Changchun University of Chinese Medicine

基　　金：吉林省科技发展计划项目(201109031)

摘　　要：目的观察参红补血颗粒对乙酰苯肼/环磷酰胺血虚证模型动物免疫调节相关因子的变化,探讨参红补血颗粒对血虚证动物免疫功能的影响及相关机制。方法采用乙酰苯肼/环磷酰胺复合因素复制血虚证动物模型,酶联免疫法检测动物血清白细胞介素-2(IL-2),白细胞介素-6(IL-6),肿瘤坏死因子(TNF)的含量变化,取脾脏,计算脾脏系数。结果参红补血颗粒各给药组动物血清中IL-2、IL-6、TNF含量均明显高于模型组(P<0.05,P<0.01),脾脏指数均低于模型组(P<0.05)。结论参红补血颗粒可增加与免疫功能有关的细胞因子的含量,降低脾脏系数,提示参红补血颗粒可能通过调节与免疫功能有关的细胞因子,改善血虚证模型动物的免疫功能。Objective To observe the changes of Shenhong Buxue Granule on the related cytokines of immunoregulation of model animal with phenylhydrazine/cyclophosphamide type of blood deficiency and to explore the effects and related mechanism of Shenhong Bttxue Granule on the immune function of animals with blood deficiency. Methods Models of animalswith blood deficiency were replicated by compound factors of phenylhydrazine/cyclophospharnide. The content changes of IL- 2,IL-6 and tumor necrosis factor （TNF） were detected by enzyme-linked immune sorbent assay（ELlSA）, the spleen was taken out and the spleen coefficient was calculated. Results The content of IL-2, IL-6 e and TNF in every drug group of Shenhong Buxue Grantde was significantly higher than those in the model group（ P 〈 0.05,0.01 ）, while the spleen coefficient in every drug group of Shenhong Buxue Granule was lower than that in the model group（ P 〈 0.05）. Conclusions Shenhong Buxue Granule can increase the content of the related cytokines of immune function, decrease the spleen coefficient, showing that Shenhong Buxue Granule may improve the immune function of model animal with blood deficiency through regulating the related cytokines of immune function.

关 键 词：参红补血颗粒 血虚证 IL-2 IL-6 TNF 脾脏系数 

分 类 号：R285.5[医药卫生—中药学]