纳米金修饰的微柱型微流控芯片用于循环肿瘤细胞检测  

Microfluidic Chip with Micropillars Modified with Gold Nanoparticles for Detecting Circulating Tumor Cells

作　　者：康勤书[1] 杨沫[1] 刘蒙蒙[1] 姜晓彤[1] 杨晓庆[2] 陈浩[1] 谢敏[3] 黄卫华[3] 

机构地区：[1]华中农业大学理学院,武汉430070 [2]华中农业大学校医院,武汉430070 [3]武汉大学化学与分子科学学院,武汉430072

出　　处：《武汉理工大学学报》2014年第7期45-49,共5页Journal of Wuhan University of Technology

基　　金：中央高校基本科研业务费专项资金资助项目(2013QC018);国家大学生创新创业计划(201310504037)

摘　　要：设计并制作了微柱型聚二甲基硅氧烷(PDMS)微流控芯片,并在通道表面组装纳米金,固定上皮细胞粘附因子(EpCAM)抗体后用于循环肿瘤细胞(Circulating Tumor Cell,CTC)检测。结果表明,纳米金成功组装在γ-氨丙基三乙氧基硅烷硅烷化的PDMS微柱表面,EpCAM抗体功能化的微流控芯片能有效捕获全血中的模型CTCs,当流速小于40μL/min时,捕获效率大于90%。建立一种简单廉价、捕获效率高的CTCs检测新方法,有望为全血中CTCs的分离提供新的策略。The PDMS microfluidic chips with micro pillars were designed and fabricated,and then gold nanoparticles were assembled on the surface of PDMS micropillars.After EpCAM antibody was immobilized,the microfluidic chips were used for the detection of model CTCs（circulating tumor cells）in the whole blood.The experimental results showed that gold nanoparticles were successfully assembled on the surface of PDMS miropillars after the microchannels were silanized byγ-aminopropyl triethoxysilane.EpCAM antibody functionalized microfluidic chip can effectively simulated CTCs,and capture efficiency is greater than 90% when the flow velocity is less than 40μL/min.A simple and cheap new method with high capture efficiency of cancer cells was established,which is likely to provide a new strategy for isolation of CTCs from the whole blood.

关 键 词：微流控芯片 PDMS微柱 纳米金 循环肿瘤细胞 

分 类 号：O652.6[理学—分析化学]