不同饮水氟浓度对大鼠生化指标影响  

Effects of different doses of fluoride in drinking water on biochemical indexes in rats

作　　者：刘璐[1,2] 张颖[1,2] 顾何锋[1,2] 张凯强[1,2] 马林[1,2] 程睿波[1,2] 张思宇[1,2] 

机构地区：[1]中国医学大学口腔医学院 [2]辽宁省口腔医学研究所口腔预防科,辽宁沈阳110002

出　　处：《中国公共卫生》2014年第11期1476-1478,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81072245);中国医学大学附属口腔医院青年科研启动基金(k101593-13-42)

摘　　要：目的建立慢性饮水型氟中毒动物模型并观察其血清生化指标的改变。方法 48只Wistar大鼠随机分成4组,染氟组分别饮用含氟化钠(50、100、150mg/L)自来水,对照组饮用自来水;8周后麻醉处死,腹主动脉取血,测定血清钙、磷、尿酸及碱性磷酸酶(ALP)活性;用氟离子选择电极法测定各组大鼠血氟及骨氟(股骨)浓度。结果与对照组比较,低、中、高剂量染氟组大鼠血氟含量[分别为(0.175±0.025)、(0.230±0.036)、(0.313±0.042)μ/mL]与骨氟含量[分别为(893.628±67.748)、(1 001.111±34.121)、(1 248.152±77.131)μg/mg]显著升高(P<0.05);与对照组比较,低、中、高剂量染氟组大鼠血清钙含量[分别为(2.212±0.282)、(2.185±0.300)、(2.148±0.179)mmol/L]下降,血清磷[分别为(2.434±0.778)、(2.478±0.246)、(2.505±0.365)mmol/L]上升,差异均有统计学意义(P<0.05);与对照组比较,高剂量染氟组大鼠尿酸含量[(47.286±1.732)μmol/L]下降,ALP活性[(92.667±11.719)U/L]上升(P<0.01)。结论在排除其他代谢性骨病条件下,血清氟、钙、磷、尿酸和ALP可以作为地氟病辅助诊断依据。Objective To establish an animal model of drinking-water endemic fluorosis and to observe the changes of serum biochemical parameters.Methods A total of 48 Wistar rats were randomly divided into four groups,including control group（distilled water）,low-dose group（NaF,50 mg/L）,medium-dose group（NaF,100mg/L）,and high-dose group（NaF,150 mg/L）（12 rats in each group）.After eight weeks,the rats were sacrificed by anesthesia and their serum samples were collected.The biochemical techniques were used to test serum calcium,phosphorus,uric acid,and activity of alkaline phosphatase（ALP）in serum of fluorosis rats.Changes in the fluorine content in serum and bone of the rats in each group were analyzed with fluoride ion selective electrode method.Results The fluorine content in serum（0.175±0.025,0.230±0.036,and 0.313±0.042μg/mL）and bone（893.628±67.748,1001.111±34.121,and1248.152±77.131μg/mg）and the level of phosphorus in serum（2.434±0.778,2.478±0.246,and2.505±0.365 mmol/L）for low-,moderate-,and high-fluoride group were significantly higher than those of the control group（all P〈0.05）and increased with the increment of fluoride concentration.The level of calcium in serum（2.212±0.282,2.185±0.300,and 2.148±0.179 mmol/L）in the low-,moderate-,and high-fluoride group were significantly lower than that of the control group（P〈0.05）.The level of uric acid in serum in high-dose group（47.286±1.732μmol/L）was significantly lower than that of the control group,and the activity of ALP in high-dose group（92.667±11.719 U/L）increased significantly.Conclusion Serum calcium,phosphorus,fluorine,uric acid and alkaline phosphatase are important indicators in auxiliary diagnosis of endemic fluorosis and could be used as early diagnosis parameters before bone morphological changes.

关 键 词：钙 磷 碱性磷酸酶(ALP) 尿酸 氟 

分 类 号：R123.1[医药卫生—环境卫生学]