巴豆醛对水稻悬浮培养细胞蛋白质表达的影响  

Effects of Crotonaldehyde on the Protein Expression of Rice Suspension Cells

作　　者：刘悦萍[1] 郭蓓[1] 胡昊[2] 张国庆[1] 

机构地区：[1]北京农学院生物科学与工程学院,北京102206 [2]北京农学院植物科学与技术学院,北京102206

出　　处：《生物技术通报》2014年第10期119-127,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31101509)

摘　　要：对巴豆醛处理水稻悬浮培养细胞后蛋白质组水平的变化进行了研究,以期发现在调控水稻细胞周期活动中起重要作用的蛋白。采用双向电泳分离蛋白,PDQuest软件分析凝胶图谱确定差异蛋白点,LCQ-Fleet ion trap系统鉴定蛋白,相关数据库检索分析差异蛋白点,获得了45个差异蛋白点,经质谱鉴定和数据库分析得到26个可信蛋白,集中在胁迫反应、蛋白质命运,信号转导,物质与能量代谢和膜功能等。在这些蛋白中,小分子量热激蛋白、Skp1蛋白和26S蛋白酶,CDC48,6-磷酸葡萄糖胺乙酰基转移酶以及蔗糖合酶等蛋白已有文献报告其在植物细胞周期调控中的作用,其它一些鉴定的蛋白参与了细胞的生理活动,其与细胞周期间的相互关系需进一步的研究。巴豆醛处理影响了水稻悬浮培养细胞正常的细胞周期活动,鉴定出部分蛋白与细胞周期的调控具有密切关系,而一些与水稻细胞分化、发育相关蛋白也得到了鉴定,其在细胞周期上的作用尚未有文献报告,这些蛋白的进一步研究将有助于揭示水稻细胞周期的调控机制。In order to study the important proteins related to cell cycle of rice, the effect of crotonaldehyde on the protein expression of suspension cell line of rice was investigated. The proteins were separated by the method of two dimensional electrophoresis, the different protein spots were analyzed by PDQuest software, and then the different protein spots were identified by LCQ-Fleet ion trap and database searching. The results showed that there were forty-five protein spots expressed differently, and twenty-six of them were identified by mass spectrometry and database analysis. The predicted function of these proteins was related to stress response, protein synthesis and processing, signal transduction, metabolism membrane function. Some of the proteins, including small molecular proteins, Skpl, 26S proteasome, cell division cycle protein 48, glucosamine-6-phosphate acetyltransferase and sucrose synthase, were related with the cell cycle process. Other identified proteins should he study further to explore its function in cell cycle of rice. The normal cell cycle activity was effected by crotonaldehyde, some of the identified proteins were related with regulation of cell cycle. Other proteins related to cell differentiation had no reports about their function to cell cycle ; these proteins should be study further to explore the mechanism of cell cycle regulation.

关 键 词：巴豆醛 水稻悬浮培养细胞 差异蛋白质组 细胞周期 

分 类 号：S511[农业科学—作物学]