盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系  

Effects of Salt Stress on Telomerase Activity in Relation to DNA Stability of Ammopiptanthus mongolicus Cells

作　　者：张徐俞[1] 王瑾瑜[2] 郑广顺[3] 张俊琦[1] 卢存福[1] 

机构地区：[1]北京林业大学生物科学与技术学院分析测试中心林木育种国家工程实验室,北京100083 [2]清华大学分析测试中心,北京100084 [3]中国科学院植物研究所,北京100093

出　　处：《生物技术通报》2014年第10期134-138,共5页Biotechnology Bulletin

基　　金：高等学校学科创新引智计划资助项目(B13007);长江学者和创新团队发展计划资助项目(IRT13047);北京市教委共建项目(101009);北京市公园管理中心课题(0710016);北京市自然科学基金项目(6112016)

摘　　要：沙冬青(Ammopiptanthus mongolicus)是亚洲中部荒漠地区的常绿阔叶灌木,是古老的第三纪孑遗植物,具有极强的抗逆能力。旨在探究盐胁迫下沙冬青细胞端粒酶活性变化与染色体DNA稳定性的关系。结果表明,在100 mmol/L NaCl的低浓度盐胁迫处理下,随着处理时间的增加,端粒酶活性没有增加,未出现明显的DNA降解现象;当在500 mmol/L高浓度盐胁迫时,处理的初期端粒酶活性迅速的增加,但随着处理时间的延长,端粒酶活性下降,此时DNA未明显降解;当移除NaCl胁迫,端粒酶活性增加1.4倍,DNA保持稳定。因此推测,在盐胁迫下,沙冬青细胞端粒酶活性的维持对避免细胞遭受不可逆损伤,保持染色体DNA稳定性具有一定的作用。Ammopiptanthus mongolicus, the evergreen broadleaf shrub indigenous to the northwest desert of China, is a residual plant of the ancient subtropical area in the Tertiary Period and has been identified as the national tertiary protection species. This research was conducted to explore the relationship between DNA damage and telomerase activity of Ammopiptanthus mongolicus cells under salt stress. The results showed that telomerase activity was increased during the first 3 d of low salt ~ 100 mmol/L NaC1 ） treatment. However, when culture ceils were treated with 500 mmol/L NaC1, telomerase activity increased rapidly at the initial stage, while declined after 2 day salt stress. Telomerase activity increased 1.4-fold in the recovery phase when 500 mmol/L NaC1 was removed from the growth medium. DNA damage was not obvious during the NaC1 treatment time or in the phase when NaC1 was removed from the growth medium. It is proposed that plants might have developed a highly efficient DNA renair system to cone with transient salt stress, and telomerase may play an imoortant role in it.

关 键 词：沙冬青 盐胁迫 端粒酶 DNA损伤 

分 类 号：S793.9[农业科学—林木遗传育种]