PFOS对东亚三角涡虫HSP70蛋白及基因表达的影响  

Effects of PFOS on Expression of HSP70 in Planarian Dugesia japonica

作　　者：宫晓宁[1] 袁佐清[1] 白芸[1] 

机构地区：[1]山东理工大学生命科学学院,淄博255049

出　　处：《生物技术通报》2014年第10期156-160,共5页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(31100377);山东省自然科学基金资助项目(ZR2011CQ018)

摘　　要：全氟辛烷磺酸(Perfluorooctane sulfonate,PFOS)是一种持续的有机污染物,是全氟化合物(Perfluorinated chemicals,PFCs)在环境中的代表性物质。以低等三胚层动物涡虫作为试验材料,使用Western blot及RT-PCR技术探究PFOS对涡虫HSP70蛋白及基因表达的影响。结果表明,PFOS短时间处理能诱导涡虫hsp70 mRNA表达量升高,涡虫再生10 d mRNA表达受到抑制,HSP70蛋白表达量在涡虫再生4 d和7 d时随PFOS浓度显著上升,其它组变化不明显,PFOS对mRNA和蛋白表达量趋势影响不一致,可能少量PFOS能抑制HSP70蛋白翻译,药物在涡虫体内积累量增加,蛋白高表达抵御毒性伤害。Perfluorooctane sulfonate （ PFOS ） is a persistent organic pollutant and has been found to be the predominant perfluorinated chemicals （ PFCs ） in the environment. In this study, we used planarians Dugesiajaponica, which belong to the phylum Platyhelminthes, class TurbeUaria, as an animal assay to evaluate the toxicological effects of PFOS on protein and mRNA expression of HSP70. The results indicated that the expression of hspTO mRNA of planarians increased exposed to PFOS in short time and decreased when planarians regenerated of lO- days. However, protein expression of HSP70 increased significantly after regenerating planarians exposed to PFOS for 4- and 7-days, and other groups were not influenced significantly. The trend of mRNA expression were inconsistent with protern. It may be that little PFOS would inhibit the translation of HSP70, and the number of HSP70 increased to protect from toxicity of PFOS as that was accumulated in planarians.

关 键 词：PFOS 三角涡虫 HSP70 RT-PCR Western BLOT 

分 类 号：X503.22[环境科学与工程—环境工程]