1株具有抗根结线虫活性的红树林土壤放线菌的分离与鉴定  

Isolation and Identification of an Actinomycetes Strain Against Rootknot Nematode from Mangrove Soil

作　　者：陈雨晴[1,2] 黄惠琴[2] 刘敏[2] 鲍时翔[2] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《生物技术通报》2014年第10期174-178,共5页Biotechnology Bulletin

基　　金：国家"973"计划项目(2013CB127500);国家自然科学基金项目(31170062);国家"948"项目(2011-G25);公益性行业科研专项(201403075);海南省重大科技项目(ZDZX2013023-1);中央级公益性科研院所基金项目(ITBB11-0302)

摘　　要：采用平板稀释法,从海南省东寨港红树林根际土壤中分离得到110株放线菌;利用24孔板液体筛选模型,筛选出具有抗线虫活性的放线菌菌株HA10401。该菌株发酵液在稀释20倍和40倍时,抗根结线虫校正死亡率分别为58.2%和52.6%。通过16S rDNA基因序列分析,菌株HA10401与链霉菌属菌株Strept omyces variabilis同源性最高(99.8%),在系统发育树上聚在同一分支内,亲缘关系最近。二者在形态特征、培养特征以及生理生化特征方面也基本一致,因此,鉴定菌株HA10401为变异链霉菌(Streptomyc es variabilis)。本研究首次报道了其抗根结线虫活性,值得进行更为深入的研究。110 Actinomycetes strains were isolated by plate dilution method and anti-root-knot nematode strain HA10401 was screened by 24-well plate fluid model from mangrove soil in Dongzhaigang National Nature Reserve, Hainan Province. While the fermentation broth was diluted by 20 times and 40 times, the mortality rates against nematode were 58.2% and 52.6%, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain HA10401 was closely related to Streptomyces variabilis with the highest similarity of 99.8%, and the two strains formed a cluster in the phylogenetic tree. The morphological, cultural and biochemical characteristics were basically accorded with Streptomyces variabilis. As a result, strain HA10401 was identified as Streptomyces variabilis, and its anti-root-knot nematode activity was first reported in this study and worth further research.

关 键 词：红树林 放线菌 鉴定 根结线虫 

分 类 号：S476[农业科学—农业昆虫与害虫防治]