γ-干扰素释放试验在结核病诊断中的临床应用  

The clinical application of gamma interferon release test in the diagnosis of tuberculosis （TB）

作　　者：兰远波[1] 李娜娜[1] 刘梅[1] 王建华[1] 彭文锋[1] 张建勇[1] 陈玲[1] 

机构地区：[1]遵义医学院附属医院呼吸二科、呼吸医学研究室,遵义贵州563003

出　　处：《世界最新医学信息文摘》2014年第28期40-40,42,共2页World Latest Medicine Information Electronic Version

基　　金：1.贵州省结核病综合防治201l协同创新中心（培育）项目2.贵州省第五批科技创新人才团队【黔科合（2012）4011】

摘　　要：目的：分析全血γ-干扰素释放试验对结核病辅助诊断价值。方法随机选取我院收治的肺部疾病患者436例，分为4组，第1组为痰涂片抗酸杆菌阳性的肺结核45例；第2组为痰涂片阴性的肺结核240例；第3组为经临床病理学或排除肺结核的非结核肺部疾病151例；第4组为对健康对照20例。用酶联免疫吸附试验（elisa）分别检测4组受试者血清γ-干扰素水平。结果第1组和第2组活动性肺结核患者血清γ-干扰素水平显著高于第3组和第4组非结核肺部疾病组和对照组，有统计学差异（P＜0.0083），敏感性分别为78.3%和83.3%，而第1组和第2组活动性肺结核患者之间血清γ-干扰素水平无统计学差异（P＞0.0083），第3组和第4组非结核肺部疾病组和对照组差异无统计学意义（P＞0.0083）。结论全血γ-干扰素释放试验可作为肺结核的重要辅助诊断技术，值得临床应用和推广。Objective to analyze diagnostic value of the whole blood gamma interferon release test on lung tuberculosis. Methods 436 patients were enrolled in the study, which were divided into four groups, one group included 45 patients of pulmonary tuberculosis with smear positive, the second group included 240 cases of pulmonary tuberculosis with smear negative, and the third group included 151 patients identiifed with other pulmonary disease besides tuberculosis. The fourth group enrolled 20 healthy volunteers. enzyme linked immunosorbent assay （elisa） method was used to detect serum level of the gamma interferon in the subjects of four groups. Results The level of serum gamma interferon of patients in the ifrst and second group with active tuberculosis were signiifcantly higher than that in non tuberculosis group and the control group, with signiifcant differences （P〈0.0083）, with diagnostic sensitivity of 78.3%and 83.3%, respectively. there were no statistically signiifcant differences in the level of serum gamma interferon of patients between the ifrst and second groups, and also between the third and fourth groups. Conclusion the whole blood gamma interferon release test could be used as important method in tuberculosis diagnosis, and it deserves to be applied in clinical promotion.

关 键 词：肺结核 潜伏感染 Γ-干扰素释放试验 

分 类 号：R446[医药卫生—诊断学]