通阳活血方对兔缺血再灌注窦房结细胞骨架微管蛋白β-tubulin的影响  

Effect of Tongyang Huoxue Recipe on Cytoskeleton Protein β-tubulin of Ischemia Reperfusion Injured Sinoatrial Node Cell in Rabbits

作　　者：彭杰[1] 刘如秀[1] 刘宇[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国中医药信息杂志》2014年第11期45-47,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81173447)

摘　　要：目的观察通阳活血方对模拟缺血再灌注损伤兔窦房结细胞骨架微管蛋白β-tubulin的影响,探讨其治疗病态窦房结综合征的机制。方法取新生乳兔窦房结细胞,以缺氧缺糖模拟缺血,以恢复氧和糖的供应模拟再灌注造成窦房结细胞损伤模型,将细胞分为空白组、模型组和通阳活血方高、中、低剂量组。治疗组给予通阳活血方相应浓度药物,空白组与模型组给予等容积培养基,采用酶标仪、激光共聚焦显微镜观察各组窦房结细胞活力、细胞骨架微管蛋白β-tubulin形态变化。结果模型组存活细胞量较空白组明显减少(P<0.01);β-tubulin裂解明显。通阳活血方高、中、低剂量组存活细胞量明显高于模型组(P<0.05),β-tubulin结构较模型组明显完整。结论通阳活血方可抑制模拟缺血再灌注引起的兔窦房结细胞损伤,其治疗病态窦房结综合征的机制可能与保护窦房结细胞活力及细胞骨架微管蛋白β-tubulin形态结构有关。Objective To observe the effects of Tongyang Huoxue Recipe on cytoskeleton proteinβ-tubulin of ischemia reperfusion injured sinoatrial node cell in rabbits;To discuss its mechanisms in the treatment of sick sinus syndrome. Methods Sinoatrial node cells were obtained from newborn rabbit. Oxygen and glucose were deprived to simulate ischemia and were restored to simulate reperfusion. Cells were divided into 5 groups. Tongyang Huoxue Recipe high-, medium-, low dose groups were given corresponding medicine （final concentrations of 100, 20, 10 μg/mL）. The normal group and model group were given equal volume of culture medium. Enzyme mark instrument and laser scanning confocal microscopy were used to observe the sinoatrial node cell activity and cytoskeleton protein β-tubulin of each group. Results Living cells of model group decreased significantly compared with normal group （P〈0.01）, and cytoskeleton proteinβ-tubulin cleavage significantly. Living cells of Tongyang Huoxue Recipe high, medium and low dose group were significantly higher than those in the model group （P〈0.05）, and β-tubulin structure remained more complete than the model group. Conclusion Tongyang Huoxue Recipe can inhibit the injury induced by ischemia reperfusion of sinoatrial node cells;the possible mechanism of Tongyang Huoxue Recipe in treating sick sinus syndrome is protecting activity and cytoskeleton proteinβ-tubulin of sinoatrial node cells.

关 键 词：通阳活血方 窦房结细胞 缺血再灌注 细胞骨架 β-tubulin蛋白 兔 

分 类 号：R285.5[医药卫生—中药学]