产后牛子宫内菌群变化规律及致病性研究  

Intrauterine Flora Changes of Postpartum Cows and Its Pathogenicity

作　　者：胡文举[1,2] 宋艳画[3] 秦佳辰 石奎林 于文浩[1] 刘远哲[1] 王新庄[1] 

机构地区：[1]河南农业大学,郑州450002 [2]河南广播电视大学,郑州450008 [3]河南农业职业学院,河南中牟451450 [4]平顶山市郏县红牛繁育中心,河南平顶山467124

出　　处：《湖北农业科学》2014年第17期4115-4119,共5页Hubei Agricultural Sciences

基　　金：河南省优质肉牛专项(11020311);河南省教育厅科学技术研究重点项目(13B230963)

摘　　要：为探索河南省郏县红牛产后子宫内菌群变化规律及各菌种的致病性与耐药性,采集21头产后健康的郏县红牛子宫内容物,进行了细菌计数、生化鉴定、致病性及药敏试验。结果表明,正常分娩后1 d牛子宫内即呈现污染状态,共分离出70株(9种)细菌,产后第8天子宫内细菌数量和种类均达到最大值,分别为(1.88±0.65)×107个/g和79株(9种),随后细菌数量和种类开始减少;产后第36天产吲哚金黄杆菌(Chryseobacterium indologenes)未分离到,第43天产吲哚金黄杆菌、乳房链球菌(Streptococcus uberis)、屎肠球菌(Enterococcus Faecium)、棒状杆菌属(Corynebacterium)4种细菌未分离到。小鼠致病性试验结果表明,大肠埃希菌(Escherichia coli)、蜡样芽孢杆菌(Bacillus cereus)、藤黄微球菌(Micrococcus luteus)、表皮葡萄球菌(Staphylococcus)对小鼠致病性较强,大肠埃希菌组小鼠腹腔攻毒后24 h内全部死亡,其余组小鼠在腹腔攻毒后2 d内全部死亡。大肠埃希菌、蜡样芽孢杆菌、藤黄微球菌、表皮葡萄球菌、产吲哚金黄杆菌均对阿莫西林和氨苄西林耐药,对环丙沙星、恩诺沙星、强力霉素、氯霉素、头孢他啶均敏感。To study the flora changes of postpartum cows and the pathogenicity of bacteria and drug resistance, the uterine contents were collected from 21 health postpartum Jiaxian redcows for bacteria counting, biochemical identification, test of pathogenicity and drug resistance. The results showed that the uterus of cows were polluted one day after delivery and 70 strains （9 species） bacteria were isolated. The quantity and species of the bacteria reached the peak value （1.88±0.65×107/g and 79 strains（9 species）, then started falling. Chryseobacterium indologenes were not isolated 36 days after delivery and 43 days after delivery. There were four bacteria including Chryseobacterium indologenes, Streptococcus parauberis, Enterococcus faecium and Corpmbacterium were not isolated. Pathogenicity test showed that E.coli, Bacillus cereus, Micrococcus luteus and Staphylococcus epidermidis had high pathogenicity for mice. The mice in E.coli group died in 24 h after attack test, and in other groups died in 2 d. E.coli, Bacillus cereus, Micrococcus luteus, Staphylococcus epidermidis and Chryseobacterium in- dologenes were sensitive to ciprofloxacin, enrofloxacin, doxycycline, chloromycetin and ceftazidime but resistant to amoxicillin and ampicillin.

关 键 词：郏县红牛 子宫内菌群 致病性 药敏试验 

分 类 号：S857.22[农业科学—临床兽医学] S858.23[农业科学—兽医学]