转Bt基因棉粉碎叶对土壤微生物群落功能多样性的影响  

Effect of Ground Leaves from Transgenic Bt Cotton on the Functional Diversity of Soil Microbial Community

作　　者：张美俊[1] 杨武德[1] 冯美臣[1] 肖璐洁[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《水土保持学报》2014年第5期228-234,共7页Journal of Soil and Water Conservation

基　　金：山西省自然科学基金项目(2012011030-2)

摘　　要：采用Biolog GN2微平板法,研究转Bt基因棉粉碎叶添加到土壤后,对土壤微生物群落功能多样性的影响。结果表明,在转Bt基因棉粉碎叶添加到土壤后,第10天和第40天的平均颜色变化率(AWCD)显著提高,表明微生物群落的代谢加快,活动强度加大;土壤微生物群落对糖类、胺类、氨基酸类和羧酸类4类碳源的优势利用顺序发生明显变化,表明土壤微生物群落结构及其功能多样性发生了一定改变;土壤微生物群落对聚合物类和其他类碳源的利用率无显著影响,但显著提高对糖类、胺类和氨基酸类碳源的利用率,并仅在第10天显著降低对羧酸类碳源的利用率,表明降解糖类、胺类和氨基酸类碳源的微生物可能是转Bt基因棉粉碎叶影响的主要土壤微生物类群。The effect of ground leaves of transgenic Bt cotton embedded into soil on the functional diversity of soil microbial community was assessed using Biolog GN2 plate.The results showed that the values of AWCD（average well color development）of all carbon sources utilized on 10 th and 40 th day was significantly increased.It indicated that the metabolisms and activities of soil microbial community were promoted after the ground leaves from transgenic Bt cotton were embedded into soil.The dominance utilization order for soil microbial community of carbohydrates,amines,amino acid and carboxylic acid carbons significantly changed after the ground leaves from transgenic Bt cotton were embedded into soil.It was demonstrated that the structure and functional diversity of soil microbial community changed.There were no significant effects on the utilization of polymers and miscellaneous carbons for soil microbial community after the ground leaves from transgenic Bt cotton were embedded into soil.But the utilization of carbohydrates,amines and amino acid carbons significantly increased on the 10 th and 40 th day,and the utilization of carboxylic acid carbons significantly reduced on the 10 th day for soil microbial community.It concluded that the microorganisms related to the degradation of carbohydrate,amines and amino acid carbon might be the major soil microbial groups influenced by the ground leaves from transgenic Bt cotton.

关 键 词：转BT基因棉 粉碎叶添加土壤 碳源 土壤微生物群落功能多样性 

分 类 号：S154[农业科学—土壤学]