中国小反刍兽疫疫情分析  

Analysis of Epidemic Chinese Peste Des Petits Ruminants

作　　者：刘永宏[1] 曹胜波[2] 赵丽[1] 李龙凯 杨龙峰 王海国[5] 廖秋萍[1] 焦海宏[1] 

机构地区：[1]塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [2]华中农业大学动物医学院,武汉430070 [3]新疆天康畜牧生物技术股份有限公司,乌鲁木齐830032 [4]北京市延庆县动物卫生监督管理局,北京102100 [5]新疆生产建设兵团第三师红旗农场生产科,新疆阿图什845350

出　　处：《西北农业学报》2014年第9期19-26,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：华中农业大学塔里木大学科研联合基金(HNTDLH1404);兵团塔里木畜牧科技重点实验室开放课题(HS2014011);新疆生产建设兵团博士资金(2012BB023);塔里木大学2014年度高等教育教学研究项目(TDGJ1409)

摘　　要：为分析中国PPRV毒株分子演变特点和疫情传播路线,从NCBI下载中国和世界其他国家全部PPRV毒株N和F基因全序列,应用分子生物学软件,并结合疫情发生的地理位置进行系统分析。结果显示,中国西藏阿里地区PPRV毒株之间核苷酸同源性为100%,其与西藏那曲地区毒株核苷酸同源性为99.9%;中国毒株与其他国家毒株F基因核苷酸序列分析显示,同源性为89.7%～98.8%,同源性最低的为科特迪瓦1989年毒株,同源性最高的为孟加拉国2010年毒株;N基因核苷酸序列分析显示,同源性为69.5%～98.5%,同源性最低的为朝鲜2002年毒株,同源性最高的为印度1995年毒株;F和N基因系统进化关系分析均显示,中国西藏3株PPRV均属于基因Ⅳ系;截止2014年3月30日,中国由西至东9省份发生该疫情。更加全面的分析结果,有待于更多PPRV流行毒株N或F基因全序列在GenBank的公布。In order to analyze the molecular evolution characteristic of peste des petits ruminants virus （PPRV） and the epidemic disseminated route in China, the complete sequences of PPRV N and F gene of strains from China and other countries were download from NCBI to do the system analysis combined with the application of molecular biology software and the outbreak locations. The results showed that the nucleotide homology of PPRV strains from All area of Tibet was 100%, the nucleo- tide homology of PPRV strains from of F gene nucleotide sequence of strai 89.7%--98.8%, the lowest was Cot and from Naqu area of Tibet was 99.9%. The analysis China and other countries showed th ire 1989 strain, the highest was Ban at the homology was gladesh 2010 strain;the analysis results of N gene nucleotide sequence showed that the homology was 69.5～--98.50/oo, the lowest was South Korea 2002 strain, the highest was India 1995 strain～ the phylogenetic analysis of F and N gene showed that three PPRV strains from Tibet belongs to the gene series IV ; by the end of March 30th, PPR have occurred in nine provinces from west to east in China. The more comprehensive analysis results need more N or F gene sequences of more PPRV strains to be published in GenBank.

关 键 词：中国 小反刍兽疫 疫情 

分 类 号：S855.3[农业科学—临床兽医学]