落叶型大丽轮枝菌T-DNA插入突变体库的构建  

Generation of T-DNA Insertional Mutant Library for Defoliating Verticillium dahliae

作　　者：韩帅[1] 胡东芳[1] Ahmed.A.ElSharawy 王春生[1] 商文静[1,2] 杨家荣[1] 胡小平[1] 

机构地区：[1]旱区作物逆境生物学国家重点实验室/西北农林科技大学植物保护学院,陕西杨凌712100 [2]棉花生物学国家重点实验室/中国农业科学院棉花研究所,河南安阳455100

出　　处：《西北农业学报》2014年第9期128-134,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31371888);棉花生物学国家重点实验室开放课题基金(CB2013A20);高等学校学科创新引智计划(B07049)

摘　　要：采用ATMT技术建立大丽轮枝菌落叶型菌株XJ2008菌株的T-DNA插入突变体文库,共获得6 043个突变体。从中随机挑选104个突变体,以野生型XJ2008菌株为参照,评价其致病性、菌落生长速率、分生孢子及微菌核的产生能力等。结果表明,有12.5%的突变体丧失产孢能力,4.8%的突变体的生长速率显著减慢,8.7%的突变体的生长速率显著加快,12.5%的突变体丧失产生微菌核的能力,47.1%的突变体的致病性显著低于野生型菌株XJ2008,且突变体2-736、2-740、2-745的病情指数分别约为野生型菌株XJ2008的0.184、0.168和0.197倍。该突变体库突变体遗传稳定性好,性状多样性丰富。T-DNA insertional mutant library of defoliating Verticillium dahliae strain XJ2008 with 6 043 mutants was established using ATMT （Agrobacterium tumefaciens-mediated transformation） technology. One hundred and four mutants selected randomly from the library were used to evaluate their pathogenesis, colony growth rate, sporulation, and microsclerotium production using wild type strain XJ2008 as reference strain. Results showed that 12. 5% of mutants lost sporulation ability, 4.8% of mutants reduced their colony growth rate, 8.7% of mutants increased their colony growth rate, 12.5~ of mutants lost their ability to produce microsclerotia, and 47. 1% of mutants reduced their pathogenesis. Especially, the disease index of mutant 2 736, 2-740, and 2-745 was 0. 184, 0. 168 and 0. 197 times of wild type strain XJ2008. The mutants in this library have genetic stability and characteristic diversity.

关 键 词：大丽轮枝菌 微菌核 突变体 致病性 

分 类 号：S432.4[农业科学—植物病理学]