TLR2和TLR4在大鼠光滑念珠菌肺部感染中的表达及意义  

Expression of TLR2 and TLR4 in the lungs of mice infected by candida glabrata

作　　者：吴晔[1] 骆雪萍[1] 吴呈霖 张雪[1] 

机构地区：[1]桂林医学院附属医院重症医学科,广西桂林541001

出　　处：《重庆医学》2014年第30期4034-4036,4039,共4页Chongqing medicine

基　　金：广西医疗卫生重点科研课题(重2012007);广西自然科学基金项目(2013GXNSFAA019209)

摘　　要：目的研究Toll样受体2(TLR2)和TLR4在光滑念珠菌肺部感染大鼠中的作用。方法将36只大鼠分为3组,每组12只:对照组(A组)、未免疫抑制肺部光滑念珠菌感染组(B组)及免疫抑制肺部光滑念珠菌感染组(C组)。每组分别在造模成功后第1、3天各处死大鼠6只,观察肺组织病理变化,用逆转录PCR(RT-PCR)法检测肺组织TLR2、TLR4mRNA的表达、酶联免疫吸附实验(ELISA)法测定TNF-α蛋白水平。结果 A组肺组织正常;B组部分肺泡塌陷、炎症细胞浸润,未见孢子或菌丝;C组大部分肺泡塌陷,部分扩张,大量炎症细胞浸润、并有孢子菌丝聚积。第1、3天TLR2mRNA和TNF-α蛋白表达水平C组明显高于其他两组(P<0.01),B组高于A组(P<0.05);C组第3天TLR2、TLR4mRNA,TNF-α表达水平高于第1天(P<0.05,P<0.01),且TLR4表达高于A组(P<0.05)。结论 TLR2和TNF-α可能参与光滑念珠菌肺部感染的发生发展,TLR4可能起协同作用。Objective To study the roles of Toll-like receptor 2（TLR2）and Toll-like receptor 4（TLR4）in the progress of pulmo-nary infection with candida glabrata in experimental mice .Methods The mice were divided into three groups ,the control group （group A） ,mice infected with candida glabrata（group B）and immunosuppressive mice infected with candida glabrata（group C） .6 mice in each group were killed at 1st and 3rd day after the success in pulmonary infection with candida glabrata .The lung tissues from each group were collected for pathological analysis and PT-PCR to detect the expression level of TLR2 and TLR4 .Protein TNF alpha（TNF-α）level was measured by ELISA method .Results The pathological analysis showed the structure was normal and there was no inflammatory reaction in lungs in group A .The infiltration of inflammatory cells ,weak injuries but no conidia or hyphae in lungs were found in group B .Most of the alveolar collapse and severe damage ,part of the expansion ,a large number of in-flammatory cells infiltration ,accompanying with conidia and hyphae accumulation were observed in group C .The expression levels of TLR2 mRNA and TNF-αprotein in mice lungs at the 1st day and the 3rd day in group C were significantly higher than in group A and B（P〈0.05） ,while group B was higher than group A（P〈0.05）;The expression levels of TLR2 ,TLR4 mRNA and TNF-αprotein in group C at the 3rd day was higher than the 1st day（P〈0.01） ,meanwhile ,the expression of TLR4 was higher than in group A（P〈0.05） .Conclusion TNF-αand TLR2 expression maybe involve in the infection and process of candida glabrata ,and TLR4 may play a synergistic effect .

关 键 词：念珠菌 光滑 TLR2 TLR4 肺组织 

分 类 号：R519.3[医药卫生—内科学]