不同产地栽培及野生喜马拉雅紫茉莉中葫芦巴碱含量测定  

Content Determination of Trigonelline in Wild and Cultivated Himalaica Mirabilis of Different Districts

作　　者：林辉[1] 赵婷[2] 仁青加[3] 邹慧琴[2] 任智宇 李佳慧[2] 彭莲[2] 闫永红[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,湖北武汉430030 [2]北京中医药大学,北京100102 [3]西藏藏医学院,西藏拉萨850000

出　　处：《中国中医药信息杂志》2014年第12期76-79,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市青年英才计划(50010402/0101324003);北京中医药大学自主选题项目(2013-ZYY-03;500102/0100605076)

摘　　要：目的 比较不同产地栽培与野生喜马拉雅紫茉莉中所含葫芦巴碱的差异.方法 采用高效液相色谱法测定喜马拉雅紫茉莉中葫芦巴碱的含量.采用ZORBAX XDB-CN 色谱柱（4.6 mm×250 mm,5 μm）;流动相：乙腈-0.03%冰醋酸（85∶15）;流速：0.8 mL/min;检测波长：265 nm,参比波长：360 nm;柱温：30 ℃.结果 以峰面积对对照品溶液浓度进行回归计算,得回归方程A =23.409C -26.398,r =0.999 8,葫芦巴碱在2.004～200.400 μg/mL 范围内与峰面积线性关系良好,葫芦巴碱的平均回收率为99.57%,RSD=1.11%.结论 不同产地栽培与野生喜马拉雅紫茉莉中葫芦巴碱含量无明显差异,本研究为栽培喜马拉雅紫茉莉药材的使用奠定了基础.Objective To compare differences and similarities of the content of trigonelline in Himalaica mirabilis of either wild or cultivated materials from different places. Methods An HPLC method was established to determine the content of trigonelline in Himalaica mirabilis. ZORBAX XDB-CN column (4.6 mm×250 mm, 5 μm) was used, with mobile phase of acetonitrile-0.03%acetic acid (85∶15), flow rate of 0.8 mL/min, detection wavelength of 265 nm, determination wavelength of 360 nm, and column temperature of 30 ℃. Results Regression calculation was made on peak area with the reference solution concentration, and then got the regression equation A=23.409C-26.398, r=0.999 8. Trigonelline showed good linear relation with peak area among the range of 2.004-200.400 μg/mL. The average recovery of trigonelline was 99.57%, RSD=1.11%. Conclusion There was no significant difference in the content of trigonelline of either wild or cultivated materials from different places. This study laid the foundation of application of the cultivated Himalaica mirabilis.

关 键 词：喜马拉雅紫茉莉 葫芦巴碱 含量测定 

分 类 号：R284.1[医药卫生—中药学]