生姜硫磺熏蒸前后HPLC特征色谱图研究  

A Study on HPLC Fingerprints of Zingiber Officinale Before and After Sulfur-fumigation

作　　者：李平[1] 蒋俊[1,2] 汤坚[3] 汪华君[3] 顾丽亚[2] 李昊天[2] 贾晓斌[2] 

机构地区：[1]镇江出入境检验检疫局国家食品添加剂及调味品检测重点实验室,江苏镇江212008 [2]江苏省中医药研究院国家中医药管理局释药系统重点研究室,江苏南京210028 [3]江苏大学附属医院药剂科,江苏镇江212001

出　　处：《中国中医药信息杂志》2014年第12期85-87,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：镇江市科技支撑计划-社会发展项目(SH2012010);江苏大学医学临床科技发展基金(JLY20120170)

摘　　要：目的对比硫磺熏蒸前后生姜高效液相色谱(HPLC)指纹图谱结构差异,探讨硫磺熏蒸对生姜质量的影响。方法采用HPLC二极管阵列检测器,ZORBAX SB-C18柱(4.6 mm×150 mm,5μm),乙腈-水为流动相,梯度洗脱,体积流量1 m L/min,柱温25℃,检测波长280 nm,建立硫磺熏蒸前后生姜的化学成分指纹图谱,对硫磺熏蒸前后的HPLC图谱进行成分分析,并采用外标法对活性成分6-姜酚进行定量检测。结果经过比较3批生姜硫熏品与3批生姜非硫熏品的指纹图谱,建立了17个共有色谱峰,3号色谱峰的峰面积下降了50.68%,10号峰下降了64.41%,11号峰下降了67.68%,17号下降了21.23%。主要活性成分6-姜酚含量无显著变化。结论生姜经过硫磺熏蒸后,其化学成分组成结构及主要活性成分含量均发生改变,其安全性、有效性有待进一步商榷。Objective To explain the differences between zingiber officinale and its sulfur fumigation products on chromatography fingerprints by HPLC-DAD;To discuss the influence of sulfur-fumigation on the quality of zingiber officinale. Methods HPLC, diode array detector, and ZORBAX SB-C18 column were used with acetonitrile-water as the mobile phase, gradient elute, volume flow rate of 1 mL/min, column temperature of 25 ℃, and detection wavelength of 280 nm. HPLC-DAD technology was applied to establish the fingerprints of zingiber officinale before and after sulfur-fumigating process, in order to analyze the HPLC fingerprints of zingiber officinale before and after sulfur-fumigating process. External standard method was used to do the quantitative determination of 6-gingerol. Results The 17 common peaks were identified through the comparison of 3 batches of fingerprints of zingiber officinale and their sulfur-fumigated samples. The peak areas of NO.3, NO.10, NO.11, and NO.17 were reduced by 50.68%, 64.41%, 67.68%, and 21.23%respectively. The content of 6-gingerol had no significant change. Conclusion The chemical composition of zingiber officinale changed at different degrees after sulfur-fumigated process. The safety and effectiveness of sulfur fumigation products of zingiber officinale require more researches.

关 键 词：生姜 硫磺熏蒸 6-姜酚 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]