竹节参多糖的免疫佐剂活性研究  

Study on Adjuvant Immunoactivity of Polysaccharides from Panax Japonicus

作　　者：崔倩倩[1] 孙志伟[2] 张长城[1] 王婷[1] 周志勇[1] 何毓敏[1] 刘朝奇[3] 袁丁[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]湖北三峡职业技术学院,湖北宜昌443002 [3]三峡大学分子生物学研究所,湖北宜昌443002

出　　处：《中国中医药信息杂志》2014年第12期88-91,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81100957;81273895;81373881);湖北省自然科学基金创新群体项目(2013CFA014)

摘　　要：目的研究不同醇沉竹节参多糖的免疫佐剂活性,探讨其中佐剂活性最强的部分。方法通过对竹节参总多糖进行不同浓度醇沉,得到30%醇沉物、60%醇沉多糖和90%醇沉多糖,将各段多糖分别与卵白蛋白混合注入小鼠体内进行免疫,每周免疫1次,共3次。最后1次免疫后5 d处死小鼠取血,测定抗体效价。对3种醇沉物质进行红外光谱扫描,初步确定多糖的类型。结果各段多糖的抗体效价与对照组比较均显著增加,尤以60%醇沉多糖最为明显。红外图谱分析表明,竹节参多糖的骨架中含有吡喃糖环结构。结论竹节参多糖的免疫佐剂效果显著,其中60%醇沉多糖的佐剂效果最强。Objective To study the adjuvant immunoactivity of polysaccharides from Panax japonicus by alcohol of different concentrations;To discuss its part with the strongest adjuvant immunoactivity. Methods Polysaccharides from Panax japonicus was sunk with alcohol of different concentrations, and 30%alcohol compound, 60%and 90%alcohol polysaccharide were obtained. Different segments of polysaccharide and OVA protein were injected to mice once a week for three times for immunity. Five days after the last immunity, the mice were executed to collect blood, and the antibody titer was determined. The three parts of alcohol compound were scanned by infrared spectrum to determine the type of polysaccharide preliminarily. Results Compared with the control group, the antibody titer of different segments of polysaccharide obviously increased, especially the polysaccharide sunk by 60%alcohol. Infrared spectrum analysis showed that polysaccharides from Panax japonicus contained pyranose ring structure. Conclusion Polysaccharides from Panax japonicus has significant adjuvant immunoactivity, and polysaccharide sunk by 60%alcohol has the strongest adjuvant effects.

关 键 词：竹节参总多糖 分段醇沉 免疫佐剂 红外图谱 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]