丙型肝炎病毒核酸和抗体检测方法在人群筛查中的比较及应用  被引量:2

Comparison of hepatitis C virus RNA and antibody detection method in population screening and its application

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作  者:朱红艳[1] 毕胜[1] 杨曦[1] 李峥[1] 徐永敏[2] 

机构地区:[1]云南省第一人民医院检验科,云南昆明650032 [2]昆明理工大学生命科学院,云南昆明650032

出  处:《国际检验医学杂志》2014年第20期2811-2812,2815,共3页International Journal of Laboratory Medicine

基  金:云南省应用基础研究昆医联合专项(2013FB198)

摘  要:目的探讨丙型肝炎病毒核酸和抗体检测方法在人群筛查中的应用。方法采用胶体金快速试验法和酶联免疫吸附试验(ELISA)检测丙型肝炎病毒(HCV)抗体,实时荧光定量PCR(RT-PCR)检测HCV-RNA病毒载量。结果 (1)539份样本中,其中266例抗体阴性,263例抗体阳性。(2)在67例HCV-RNA病毒载量小于103 IU/mL组中,ELISA法检测HCV抗体阳性有60例,胶体金快速试验法检测抗体阳性有30例。208例HCV-RNA病毒载量大于或等于103 IU/mL组中,ELISA法检测的抗体阳性有199例,胶体金快速法检测阳性的有181例,另外有6例两种抗体检测均为阴性患者RNA病毒载量大于或等于103 IU/mL。(3)对208例HCV-RNA病毒载量大于或等于103 IU/mL样本分成4个组。GGT、ALT及AST在4组间差异均有统计学意义(P<0.05),而ALB及S/CO值在4组间差异无统计学意义(P>0.05)。结论在人群筛查中为了减少漏诊率,尽早诊断丙型肝炎,要联合运用以上各实验室检测方法,综合分析。Objective To investigate the application of hepatitis C virus RNA and antibody detection method in population screening.Methods The colloidal gold rapid test method and the enzyme-linked immunosorbent assay (ELISA)were adopted to detect hepatitis C virus (HCV)antibodies,and the real-time quantitative PCR (RT-PCR)was adopted to detect HCV-RNA viral load.Results (1)Among 539 samples,266 cases were antibody negative and 263 cases were antibody positive.(2)Among 67 cases in the HCV-RNA viral load 〈103 IU/mL group,60 cases were HCV antibody positive by ELISA and 30 cases were HCV antibody positive by colloidal gold rapid test.Among 208 cases in the HCV-RNA viral load ≥ 103 IU/mL,199 cases were antibody positive by ELISA,but only 181cases were antibody positive by the colloidal gold rapid method.Other 6 cases of were 2 kinds of antibody negative had the HCV-RNA viral load ≥ 103 IU/mL.(3)208 cases of HCV-RNA viral load ≥ 103 IU/mL sample were divided in-to four groups.GGT,ALT and AST were statistically significantly different P 〈0.05),while ALB and S/CO values hadno statisti-cal difference (P 〉0.05).Conclusion In order to reduce the missed diagnosis rate and diagnose hepatitis C as early as possible,the above laboratory detection methods should be jointly applied and the comprehensive analysis should be conducted in population screening.

关 键 词:丙型肝炎病毒抗体 丙型肝炎病毒RNA 酶联免疫吸附试验 实时荧光定量PCR 

分 类 号:R446.6[医药卫生—诊断学]

 

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