骨形态发生蛋白-2缓释复合物的制备及其性能的研究  

Preparation and Osteoinduction Capacity of gradual release carrier for BMP-2 Composites

作　　者：李承靖[1] 后军[2] 夏荣[1] 方冬冬[1] 杨文宇[1] 肖峰[1] 

机构地区：[1]安徽医科大学第二附属医院口腔科,合肥230061 [2]安徽医科大学第一附属医院口腔颌面外科,合肥230001

出　　处：《安徽卫生职业技术学院学报》2014年第5期97-98,100,共3页Journal of Anhui Health Vocational & Technical College

基　　金：安徽医科大学校科研基金项目(编号:2011xkj045);安徽省科技厅2013年度重点科技项目(编号:1301043050)

摘　　要：目的：研究BMP-2缓释复合物的成骨性能，并对比其与单纯BMP-2间成骨差异。方法：该实验采用藻酸钠、BMP-2和氯化钙混合制备藻酸钙-BMP复合材料，进行体外观察和兔动物试验，通过用x线、组织学、骨密度等方法，研究其诱导成骨活性。结果：缓释组和BMP－2组均在4～12周形成新生骨组织，两组标本均为编织骨形态，x线、组织学、骨密度等方法，均提示两组在骨新骨形成量上存在显著差异，缓释组新骨形成量大于单纯BMP-2组。结论：BMP－2缓释载体复合物刺激成骨与直接应用BMP－2刺激成骨对新骨形成量存在显著差异，缓释复合物可以持续稳定促进新骨形成，达到缩短骨愈合的目的。Objective：To evaluate the osteoinduction capacity of gradual release canner for BMF-2 composites, and Comparison between only BMP-2 and gradual release cartier for BMP-2 in repairing bone defects. Methods：Cal- cium alginate/BMP composites were prepared with sodium alginate, bone morphogenetic protein-2 （BMP-2） and cal- cium chloride. The composites were observed in vitro and tested with ectopic bone formation, compared with the BMP-2. The osteoinduction capacity was evaluated by X-ray, histological exam ination, biomechanics analysis, and bone density measurement. Results：Bone induced by CaAG-BMP-2 and BMP-2 composites were both found as wo- ven bone between 4 and 12 weeks; The results of X-ray, histological exam ination, biomechanics analysis, and bone density measurement revealed CaAG-BMP-2 were much better than those in BMP-2 groups. There were much differences between these two groups in the amount of bone formation. Conclusion：Newly-formed bone can be induced by the composites of calcium alginate/BMP, more than only BMP-2 groups. Gradual release carrier for BMP-2 com- posites can be sustained and stable to promote new bone formation, and shorten the bone healing.

关 键 词：骨形态发生蛋白 藻酸钠 缓释 

分 类 号：R686.5[医药卫生—骨科学]